Anti-PHLPP2 抗体 - N-terminal (ab153918)
Key features and details
- Rabbit polyclonal to PHLPP2 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PHLPP2 antibody - N-terminal
PHLPP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PHLPP2 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to a region within N terminal amino acids 13-234 of Human PHLPP2.
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ポジティブ･コントロール
- Mouse brain whole cell lysate; A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153918の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 147 kDa.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 147 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. -
組織特異性
In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). -
配列類似性
Contains 22 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain. -
細胞内局在
Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23035 Human
- Entrez Gene: 244650 Mouse
- Omim: 611066 Human
- SwissProt: Q6ZVD8 Human
- SwissProt: Q8BXA7 Mouse
- Unigene: 709458 Human
- Unigene: 23494 Mouse
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別名
- KIAA0931 antibody
- PH domain and leucine rich repeat protein phosphatase 2 antibody
- PH domain leucine rich repeat protein phosphatase like antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PHLPP2 antibody - N-terminal (ab153918)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PHLPP2 with ab153918 at 1/500 dilution. Lower image is co-stained with Hoechst 33342.
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Western blot - Anti-PHLPP2 antibody - N-terminal (ab153918)Anti-PHLPP2 antibody - N-terminal (ab153918) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 147 kDa
5% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab153918 は論文での使用が確認できていません。