Anti-PHLDA3 抗体 (ab22822)
Key features and details
- Goat polyclonal to PHLDA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PHLDA3 antibody
PHLDA3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PHLDA3 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide:
RARQSLGTGTLVS
, corresponding to C terminal amino acids 115-127 of Human PHLDA3. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab22822 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22822の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
A 1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. -
組織特異性
Widely expressed with lowest expression in liver and spleen. -
配列類似性
Belongs to the PHLDA3 family.
Contains 1 PH domain. -
ドメイン
The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23612 Human
- Entrez Gene: 27280 Mouse
- Entrez Gene: 363989 Rat
- Omim: 607054 Human
- SwissProt: Q9Y5J5 Human
- SwissProt: Q9WV95 Mouse
- SwissProt: Q5PQT7 Rat
- Unigene: 268557 Human
see all -
別名
- PHLA3_HUMAN antibody
- PHLDA 3 antibody
- phlda3 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab22822 は 4 報の論文で使用されています。
- Bensellam M et al. Phlda3 regulates beta cell survival during stress. Sci Rep 9:12827 (2019). PubMed: 31492921
- Leszczynska KB et al. Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT. J Clin Invest 125:2385-98 (2015). PubMed: 25961455
- Ohki R et al. PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A 111:E2404-13 (2014). PubMed: 24912192
- Kawase T et al. PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt. Cell 136:535-50 (2009). WB . PubMed: 19203586