AB22822

Anti-PHLDA3 抗体

Name: Anti-PHLDA3 抗体 (ab22822) | Abcam
Brand: Abcam Limited
SKU: AB22822
Price: 75000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-PHLDA3 antibody

(1 Review)

(5 Publications)

Goat Polyclonal PHLDA3 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PHLDA3 aa 100 to C-terminus.

別名を表示する

TIH1, PHLDA3, Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homolog 1

1 Images

Unknown

Western blot - Anti-PHLDA3 antibody (AB22822)

Lanes 1 - 2:

Western blot - Anti-PHLDA3 antibody (ab22822) at 1 µg/mL

Lane 3:

Anti-myc tag antibody at 1/1000 dilution

Lanes 1 and 3:

HEK293 lysate overexpressing Human PHLDA3 in RIPA buffer at 10 µg

Lane 2:

Mock-transfected HEK293

Predicted band size: 14 kDa

true

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human PHLDA3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5J5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab22822 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHLDA3 also known as PHLDA family member 3 is a small protein with a molecular mass of about 15 kDa. It functions mechanically as an antagonist of the AKT signaling pathway. PHLDA3 can inhibit AKT by binding to its pleckstrin homology (PH) domain preventing AKT's interaction with other molecules. This protein is expressed in various tissues throughout the body including the brain lungs and liver indicating its role in diverse cellular functions.

Biological function summary

The protein acts as a tumor suppressor through its ability to regulate cell growth and apoptosis. PHLDA3 does this by modulating the AKT pathway inhibiting cell survival and promoting apoptosis. It is not part of a larger complex but interacts directly with other proteins to execute its function. The influence of PHLDA3 on cell cycle processes suggests it plays roles in maintaining normal cellular functions and preventing uncontrolled proliferation.

Pathways

Several studies have shown PHLDA3 integrates into the p53 signaling pathway a critical pathway for cellular stress responses and tumor suppression. It interacts with the AKT pathway which connects it to proteins like PI3K and PTEN influencing cellular growth and apoptosis. PHLDA3 effectively bridges interaction between the p53 pathway and AKT pathway which balances cell fate decisions under stress conditions.

PHLDA3 exhibits a significant impact on cancer development and progression. Loss of PHLDA3 expression has been observed in various tumors suggesting its role in cancer development. Additionally it relates to diabetes where altered AKT signaling affects insulin response. In cancer PHLDA3 connects to the tumor suppressor protein p53 influencing its regulatory functions while in diabetes its relation to AKT pathway points to disturbances in glucose homeostasis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.

See full target information PHLDA3

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 21:613 PubMed33936270

2021

PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Ying Chen,Fen Ai,Yun-Qian Li,Kun Zhang,Wei-Tong Zhang

Scientific reports 9:12827 PubMed31492921

2019

Phlda3 regulates beta cell survival during stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Bensellam,Jeng Yie Chan,Kailun Lee,Mugdha V Joglekar,Anandwardhan A Hardikar,Thomas Loudovaris,Helen E Thomas,Jean-Christophe Jonas,D Ross Laybutt

The Journal of clinical investigation 125:2385-98 PubMed25961455

2015

Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna B Leszczynska,Iosifina P Foskolou,Aswin G Abraham,Selvakumar Anbalagan,Céline Tellier,Syed Haider,Paul N Span,Eric E O'Neill,Francesca M Buffa,Ester M Hammond

Proceedings of the National Academy of Sciences of 111:E2404-13 PubMed24912192

2014

PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Rieko Ohki,Kozue Saito,Yu Chen,Tatsuya Kawase,Nobuyoshi Hiraoka,Raira Saigawa,Maiko Minegishi,Yukie Aita,Goichi Yanai,Hiroko Shimizu,Shinichi Yachida,Naoaki Sakata,Ryuichiro Doi,Tomoo Kosuge,Kazuaki Shimada,Benjamin Tycko,Toshihiko Tsukada,Yae Kanai,Shoichiro Sumi,Hideo Namiki,Yoichi Taya,Tatsuhiro Shibata,Hitoshi Nakagama

Cell 136:535-50 PubMed19203586

2009

PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.

Applications

Species

Unspecified reactive species

Tatsuya Kawase,Rieko Ohki,Tatsuhiro Shibata,Shuichi Tsutsumi,Naoko Kamimura,Johji Inazawa,Tsutomu Ohta,Hitoshi Ichikawa,Hiroyuki Aburatani,Fumio Tashiro,Yoichi Taya

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHLDA3 抗体

Anti-PHLDA3 antibody

Western blot - Anti-PHLDA3 antibody (AB22822)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise