JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB196757

Anti-PHLDA3 抗体

Anti-PHLDA3 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal PHLDA3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PHLDA3.

別名を表示する

TIH1, PHLDA3, Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homolog 1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA3 antibody (AB196757)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLDA3 antibody (AB196757)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PHLDA3 with ab196757 at 1/50 dilution (left) and synthesized peptide (right).

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Synthetic Peptide within Human PHLDA3. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5J5

style
left-column

代替製品

Primary Antibodies

AB81464

Anti-PHLDA3 antibody [4B6]

Western blot - Anti-PHLDA3 antibody [4B6] (AB81464)

  • 0
  • 0
Primary Antibodies

AB22822

Anti-PHLDA3 antibody

Western blot - Anti-PHLDA3 antibody (AB22822)

  • 4
  • 1

組合せ製品

Primary Antibodies

AB243638

Anti-CARM1 antibody [EPR23678-113]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (AB243638)

  • 0
  • 0
Primary Antibodies

AB217678

Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

20ul selling size|BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad21 antibody [EPR22506-15] - ChIP Grade (AB217678)

  • 4
  • 2
Primary Antibodies

AB128873

Anti-CTCF antibody [EPR7314(B)] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (AB128873)

  • 5
  • 1
Primary Antibodies

AB281584

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001]

20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody [RM1001] (AB281584)

  • 5
  • 4
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
ab196757 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHLDA3 also known as PHLDA family member 3 is a small protein with a molecular mass of about 15 kDa. It functions mechanically as an antagonist of the AKT signaling pathway. PHLDA3 can inhibit AKT by binding to its pleckstrin homology (PH) domain preventing AKT's interaction with other molecules. This protein is expressed in various tissues throughout the body including the brain lungs and liver indicating its role in diverse cellular functions.
Biological function summary

The protein acts as a tumor suppressor through its ability to regulate cell growth and apoptosis. PHLDA3 does this by modulating the AKT pathway inhibiting cell survival and promoting apoptosis. It is not part of a larger complex but interacts directly with other proteins to execute its function. The influence of PHLDA3 on cell cycle processes suggests it plays roles in maintaining normal cellular functions and preventing uncontrolled proliferation.

Pathways

Several studies have shown PHLDA3 integrates into the p53 signaling pathway a critical pathway for cellular stress responses and tumor suppression. It interacts with the AKT pathway which connects it to proteins like PI3K and PTEN influencing cellular growth and apoptosis. PHLDA3 effectively bridges interaction between the p53 pathway and AKT pathway which balances cell fate decisions under stress conditions.

PHLDA3 exhibits a significant impact on cancer development and progression. Loss of PHLDA3 expression has been observed in various tumors suggesting its role in cancer development. Additionally it relates to diabetes where altered AKT signaling affects insulin response. In cancer PHLDA3 connects to the tumor suppressor protein p53 influencing its regulatory functions while in diabetes its relation to AKT pathway points to disturbances in glucose homeostasis.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.
See full target information PHLDA3
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32717899

2020

Characteristics of Circular RNA Expression Profiles of Porcine Granulosa Cells in Healthy and Atretic Antral Follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Li Meng,Katja Teerds,Jian Tao,Hengxi Wei,Marcel Jaklofsky,Zhihong Zhao,Yaodi Liang,Li Li,Chi Chiu Wang,Shouquan Zhang

The Prostate 78:257-265 PubMed29250809

2017

Prostate tumors downregulate microseminoprotein-beta (MSMB) in the surrounding benign prostate epithelium and this response is associated with tumor aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Halin Bergström,Helena Järemo,Maria Nilsson,Hanibal Hani Adamo,Anders Bergh
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com