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Anti-PHLDA3 抗体 [4B6] (ab81464)

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Western blot - Anti-PHLDA3 antibody [4B6] (ab81464)

    Key features and details

    • Mouse monoclonal [4B6] to PHLDA3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2b

    製品の概要

    • 製品名

      Anti-PHLDA3 antibody [4B6]
      PHLDA3 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [4B6] to PHLDA3

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat

    • 免疫原

      Synthetic peptide: MTAAATATVL KEGVLEKRSG GLLQLWKRKR C, corresponding to N terminal amino acids 1-31 of human PHLDA3

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • MDA-MB-468 cells

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      pH: 6
      Constituents: 50% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...

    • 精製度

      Ion Exchange Chromatography

    • 特記事項（精製）

      The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. The antibody is filter sterilized.

    • ポリ/モノ

      モノクローナル

    • クローン名

      4B6

    • アイソタイプ

      IgG2b

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab81464の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
    特記事項
    WB
    Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.

    ターゲット情報

    • 機能

      p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor.

    • 組織特異性

      Widely expressed with lowest expression in liver and spleen.

    • 配列類似性

      Belongs to the PHLDA3 family.
      Contains 1 PH domain.

    • ドメイン

      The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.

    • 細胞内局在

      Cytoplasm. Membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • PHLA3_HUMAN antibody
      • PHLDA 3 antibody
      • phlda3 antibody
      • Pleckstrin homology like domain family A member 2 antibody
      • Pleckstrin homology like domain family A member 3 antibody
      • Pleckstrin homology-like domain family A member 3 antibody
      • TDAG51/Ipl homolog 1 antibody
      • TDAG51/Ipl homologue 1 antibody
      • TIH 1 antibody
      • TIH1 antibody
      see all

    画像

    • Western blot - Anti-PHLDA3 antibody [4B6] (ab81464)
      Western blot - Anti-PHLDA3 antibody [4B6] (ab81464)
      Western blotting was performed using ab81464 to detect PHLDA3 protein expression. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).

    参考文献 (2)

    ab81464 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab81464 は 2 報の論文で使用されています。

    • Wang P  et al. Pleckstrin homology-like domain family A, member 3, a miR-19a-3p-regulated gene, suppresses tumor growth in osteosarcoma by downregulating the Akt pathway. Bioengineered 13:3993-4009 (2022). PubMed: 35112982
    • Prudovsky I  et al. Phosphatidylserine colocalizes with epichromatin in interphase nuclei and mitotic chromosomes. Nucleus 3:200-10 (2012). PubMed: 22555604

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