AB194112

Anti-PHKG1 抗体 [EPR14812]

Anti-PHKG1 antibody [EPR14812]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal PHKG1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 4 publications.

別名を表示する

PHKG, PHKG1, PHK-gamma-M, Phosphorylase kinase subunit gamma-1, Serine/threonine-protein kinase PHKG1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (AB194112)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (AB194112)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PHKG1 with ab194112 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (AB194112)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PHKG1 with ab194112 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] (AB194112)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] (AB194112)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% tritonX-100 permeabilized A673 cells labeling PHKG1 with ab194112 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/400 dilution. Nuclear counter stain is DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (AB194112)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling PHKG1 with ab194112 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PHKG1 antibody [EPR14812] (AB194112)

All lanes:

Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/20000 dilution

All lanes:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 40 kDa

false

Supplier Data

Western blot - Anti-PHKG1 antibody [EPR14812] (AB194112)

All lanes:

Western blot - Anti-PHKG1 antibody [EPR14812] (ab194112) at 1/1000 dilution

Lane 1:

Mouse muscle tissue lysate at 10 µg

Lane 2:

Rat muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 40 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14812

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHKG1 is also known as phosphorylase kinase gamma 1. PHKG1 is an enzyme that plays a role in phosphorylase kinase activity. This enzyme has a molecular mass of approximately 45 kDa. Researchers found PHKG1 expressed mainly in skeletal muscle tissue where it participates in glycogen metabolism. The enzyme contains catalytic subunits that function in phosphorylating serine and threonine residues essential for glycogen breakdown.

Biological function summary

PHKG1 acts as a critical component of the larger phosphorylase kinase complex. This complex promotes glycogenolysis the process of converting glycogen to glucose-1-phosphate facilitating energy release in cells. Within tissues PHKG1 interacts with other subunits of phosphorylase kinase playing a regulatory role. Effective activation of phosphorylase b to active phosphorylase a form relies on efficient PHKG1 function.

Pathways

PHKG1 is essential in the glycogenolysis pathway. It interacts with AMP-activated protein kinase (AMPK). Besides PHKG1 associates with cAMP-dependent protein kinase (PKA) as both enzymes participate in regulating energy homeostasis. These interactions highlight the enzyme’s function in maintaining metabolic balance under stress conditions.

PHKG1 associates with McArdle's disease a glycogen storage disorder characterized by muscle weakness and fatigue. Aberrant PHKG1 activity can disrupt glycogen breakdown aggravating symptoms of the condition. Moreover metabolic syndrome a cluster of metabolic disorders connects to PHKG1 through its role in regulating glucose metabolism and shares common pathways with AMPK relating PHKG1 to potential targets for therapy in metabolic disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. In vitro, phosphorylates PYGM, TNNI3, MAPT/TAU, GAP43 and NRGN/RC3 (By similarity).

See full target information PHKG1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15554 PubMed40319072

2025

On the utility of immobilized phenylarsine oxide in the study of redox sensitive cardiac proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Asvi Arora Francois,Xiaoke Yin,Shinichi Oka,Junichi Sadoshima,Manuel Mayr,Philip Eaton

Journal of translational medicine 23:367 PubMed40128847

2025

Sodium tanshinone IIA sulfonate promotes proliferation and differentiation of endogenous neural stem cells to repair rat spinal cord injury via the Notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqing Zhong,Luchun Xu,Guozheng Jiang,Yushan Gao,Jiawei Song,Yukun Ma,Guanlong Wang,Jiaojiao Fan,Wenhao Li,Shibo Zhou,Yongdong Yang,Xing Yu

Antioxidants (Basel, Switzerland) 13: PubMed39199268

2024

Avicularin Attenuated Lead-Induced Ferroptosis, Neuroinflammation, and Memory Impairment in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Tao Guo,Chao Cheng,Jia-Xue Shi,Wen-Ting Zhang,Han Sun,Chan-Min Liu

Carbohydrate polymers 243:116435 PubMed32532388

2020

Metformin and Berberine suppress glycogenolysis by inhibiting glycogen phosphorylase and stabilizing the molecular structure of glycogen in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocui Liu,Kaiping Wang,Jing Zhou,Mitchell A Sullivan,Yage Liu,Robert G Gilbert,Bin Deng

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHKG1 抗体 [EPR14812]

Anti-PHKG1 antibody [EPR14812]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (AB194112)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (AB194112)

Immunocytochemistry/ Immunofluorescence - Anti-PHKG1 antibody [EPR14812] (AB194112)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHKG1 antibody [EPR14812] (AB194112)

Western blot - Anti-PHKG1 antibody [EPR14812] (AB194112)

Western blot - Anti-PHKG1 antibody [EPR14812] (AB194112)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise