Anti-PHIP 抗体 (ab86244)
Key features and details
- Rabbit polyclonal to PHIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PHIP antibody
PHIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PHIP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
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免疫原
Synthetic peptide corresponding to Human PHIP aa 1600-1700.
Database link: Q8WWQ0 -
ポジティブ･コントロール
- Whole cell lysate from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86244の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 230 kDa (predicted molecular weight: 207 kDa).
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 230 kDa (predicted molecular weight: 207 kDa). |
ターゲット情報
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関連性
PHIP contains two bromo domains and eight WD repeats. It may be involved in insulin signaling. It is expressed in myeloma and epidermoid carcinoma cell lines. -
参照データベース
- Entrez Gene: 55023 Human
- Entrez Gene: 83946 Mouse
- Entrez Gene: 315843 Rat
- Omim: 612870 Human
- SwissProt: Q8WWQ0 Human
- SwissProt: Q8VDD9 Mouse
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別名
- DCAF14 antibody
- DDB1 and CUL4 associated factor 14 antibody
- FLJ20705 antibody
see all
画像
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Western blot - Anti-PHIP antibody (ab86244)All lanes : Anti-PHIP antibody (ab86244) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 207 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab86244 は 5 報の論文で使用されています。
- Jo JH et al. RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter. Cell Commun Signal 21:219 (2023). PubMed: 37612584
- Su W et al. circPHIP promotes oral squamous cell carcinoma progression by sponging miR-142-5p and regulating PHIP and ACTN4 expression. Mol Ther Nucleic Acids 23:185-199 (2021). PubMed: 33376626
- Townsend A et al. DCAF14 promotes stalled fork stability to maintain genome integrity. Cell Rep 34:108669 (2021). PubMed: 33503431
- Marenne G et al. Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription. Cell Metab 31:1107-1119.e12 (2020). PubMed: 32492392
- McLure KG et al. RVX-208, an inducer of ApoA-I in humans, is a BET bromodomain antagonist. PLoS One 8:e83190 (2013). PubMed: 24391744