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AB245589

Anti-PHF8 抗体

Anti-PHF8 antibody

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(1 Publication)

Rabbit Polyclonal PHF8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PHF8 aa 900-1000.

別名を表示する

KIAA1111, ZNF422, PHF8, Histone lysine demethylase PHF8, PHD finger protein 8, [histone H3]-dimethyl-L-lysine(36) demethylase PHF8, [histone H3]-dimethyl-L-lysine(9) demethylase PHF8

1 Images
Western blot - Anti-PHF8 antibody (AB245589)
  • WB

Supplier Data

Western blot - Anti-PHF8 antibody (AB245589)

All lanes:

Western blot - Anti-PHF8 antibody (ab245589) at 0.04 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (RIPA buffer) whole cell lysate at 50 µg

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 117 kDa

false

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human PHF8 aa 900-1000. The exact immunogen used to generate this antibody is proprietary information.

Q9UPP1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
ab245589 was affinity purified using an epitope specific to PHF8 immobilized on solid support.
バッファー組成
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHF8 also known as Plant Homeodomains Finger Protein 8 is a histone demethylase enzyme. It specifically removes methyl groups from histone H3 at lysine 9 (H3K9me2/me1) and at lysine 27 (H3K27me2/me1). PHF8 is a protein with a molecular mass of around 109 kDa. This protein is expressed widely in various tissues appearing notably in brain tissues and the testis which suggests a broad physiological role.
Biological function summary

PHF8 plays an important role in the regulation of gene expression through chromatin remodeling. It forms part of transcriptional complexes and directly interacts with transcription factors and chromatin proteins. Its activity modulates gene silencing and activation processes impacting multiple cellular functions such as cell cycle progression and neurodevelopment. The activities controlled by PHF8 make it critical for maintaining normal cellular function.

Pathways

PHF8 takes part in integral signaling and epigenetic regulation pathways. It is involved in the MAPK/ERK signaling pathway which affects cell proliferation and differentiation. PHF8 also connects with other proteins such as p53 influencing pathways that govern cell growth and apoptosis. Through these interactions PHF8 helps maintain proper balance in cell cycle and development processes.

Mutations or dysregulation of PHF8 have links to developmental disorders and certain cancers. Specifically PHF8 mutations have associations with X-linked mental retardation and cleft lip/palate conditions. Dysregulated PHF8 activity interacts with proteins like p53 to influence cancer progression particularly in prostate cancer. Understanding PHF8's role aids in the exploration of therapeutic targets for these conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity : has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3. Positively modulates transcription of histone demethylase KDM5C, acting synergistically with transcription factor ARX; synergy may be related to enrichment of histone H3K4me3 in regulatory elements.
See full target information PHF8
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 19:4103-4120 PubMed38736658

2024

Long-Term Accumulation, Biological Effects and Toxicity of BSA-Coated Gold Nanoparticles in the Mouse Liver, Spleen, and Kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Jakic,Michal Selc,Filip Razga,Veronika Nemethova,Petra Mazancova,Filip Havel,Michal Sramek,Monika Zarska,Jan Proska,Vlasta Masanova,Iveta Uhnakova,Peter Makovicky,Marta Novotova,Vit Vykoukal,Andrea Babelova
View all publications
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