AB264292

Anti-PHF23 抗体

Name: Anti-PHF23 抗体 (ab264292) | Abcam
Brand: Abcam Limited
SKU: AB264292
Price: 75000 JPY
Availability: InStock

Anti-PHF23 antibody

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(1 Publication)

Rabbit Polyclonal PHF23 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PHF23 aa 350 to C-terminus.

別名を表示する

PHD finger protein 23, PDH-containing protein JUNE-1, PHF23

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF23 antibody (AB264292)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF23 antibody (AB264292)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PHF23 with ab264292 at 1/1,000 dilution. Detection : DAB.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Supplier Data

Immunoprecipitation - Anti-PHF23 antibody (AB264292)

PHF23 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264292 at 10 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264292 at 0.4 μg/ml.

Lane 1 : ab264292 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 30 secs.

All lanes:

Immunoprecipitation - Anti-PHF23 antibody (ab264292)

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-PHF23 antibody (AB264292)

All lanes:

Western blot - Anti-PHF23 antibody (ab264292) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 44 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P, IP

applications

免疫原

Synthetic Peptide within Human PHF23 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BUL5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHF23 also known as Plant Homeodomain Finger 23 is a protein involved in chromatin dynamics and regulation of gene expression. It has a molecular mass of approximately 43 kDa. PHF23 contains a zinc finger domain which is important for its role in binding to histones. The protein expresses in various tissues but shows higher expression levels in certain organs like the brain and testes. Its expression pattern suggests an involvement in critical regulatory processes.

Biological function summary

PHF23 plays a role in the modification of chromatin structure influencing gene transcription. The protein participates in the formation of a complex that is involved in transcriptional repression. It particularly associates with components of the nucleosome acting as a mediator in processes that alter the accessibility of DNA for transcription factors and enzymes. The protein's ability to bind to chromatin allows it to directly impact the epigenetic state of cells.

Pathways

PHF23 is a part of the chromatin remodeling pathway and is involved in the regulation of gene transcription. Its interaction with methylated histones connects it to the pathway of histone modification. It works alongside other proteins like LSD1 which is a lysine-specific demethylase and this collaborative function influences the transcriptional output of the cell. The protein’s actions contribute to maintaining the balance between gene activation and repression which is essential for normal cellular functions.

PHF23 relates to conditions involving abnormal chromatin remodeling such as certain cancers. Its alteration affects the typical transcriptional control which can lead to tumorigenesis. The protein interacts with other proteins including members of the histone demethylase family like KDM1A influencing pathways that become dysregulated in cancerous states. Studies suggest that understanding PHF23's exact role offers potential therapeutic targets for diseases where chromatin misregulation plays an important role.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria.

See full target information PHF23

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:558 PubMed37626047

2023

PHF23 promotes NSCLC proliferation, metastasis, and chemoresistance via stabilization of ACTN4 and activation of the ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Cheng,Hongyi Cao,Peifeng Yao,Jingqian Guan,Peihong Wu,Hairu Ji,Siyu Jiang,Yinan Yuan,Lin Fu,Qianqian Zheng,Qingchang Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHF23 抗体

Anti-PHF23 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF23 antibody (AB264292)

Immunoprecipitation - Anti-PHF23 antibody (AB264292)

Western blot - Anti-PHF23 antibody (AB264292)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise