AB129495

Anti-PHF16 抗体

Name: Anti-PHF16 抗体 (ab129495) | Abcam
Brand: Abcam Limited
SKU: AB129495
Price: 74000 JPY
Availability: InStock

Anti-PHF16 antibody

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(1 Publication)

Rabbit Polyclonal PHF16 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human JADE3 aa 750 to C-terminus.

別名を表示する

KIAA0215, PHF16, JADE3, Protein Jade-3, Jade family PHD finger protein 3, PHD finger protein 16

2 Images

Unknown

Immunoprecipitation - Anti-PHF16 antibody (AB129495)

Detection of PHF16 by Western Blot of Immunprecipitate.
ab129495 at 1 µg/ml staining PHF16 in HeLa whole cell lysate immunoprecipitated using ab129495 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : control IgG.
Predicted band size : 94 kDa.
Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PHF16 antibody (ab129495)

Predicted band size: 94 kDa

false

Unknown

Western blot - Anti-PHF16 antibody (AB129495)

All lanes:

Western blot - Anti-PHF16 antibody (ab129495) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 94 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human JADE3 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92613

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab129495 was affinity purified using an epitope specific to PHF16 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHF16 also known as JADE3 is a protein with a mass of approximately 90 kDa. It is a member of the plant homeodomain finger family of proteins. PHF16 contains domains that mediate interactions with other proteins indicating its role in chromatin remodeling and transcription regulation. This protein is widely expressed in tissues including brain liver and lung. Its localization in these tissues suggests its involvement in various cellular processes.

Biological function summary

PHF16 interacts with a range of molecular complexes related to gene expression regulation. It is a component of the HBO1 complex which plays a role in histone acetylation. This complex modifies chromatin structure to facilitate access to DNA for transcription machinery. PHF16 acts by binding to specific histone marks participating in the regulation of transcriptional activation of genes. These functions make it an important player in maintaining proper gene expression levels across diverse cell types.

Pathways

PHF16 influences transcriptional regulation pathways and epigenetic modification pathways. Within these frameworks it closely interacts with other proteins like HBO1 and ING5. PHF16 contributes to the modification of histone acetylation patterns which is important for transcriptional regulation. It assists in setting the epigenetic landscape that determines the transcriptional output of genes involved in cell cycle and development.

Altered expression or mutation of PHF16 links to several pathological conditions including cancer and developmental disorders. For example dysregulation of PHF16 expression may contribute to tumorigenesis particularly in breast cancer due to its role in regulating genes essential for cell proliferation. Moreover interactions with proteins like HBO1 and ING5 highlight its importance in pathways disrupted in developmental diseases where epigenetic misregulation plays a significant role. Understanding PHF16's function and interactions provides critical insights into its potential as a therapeutic target in these disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity.

See full target information JADE3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:8157 PubMed28811661

2017

Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mercedes Pardo,Lu Yu,Shihpei Shen,Peri Tate,Daniel Bode,Blake L Letney,Dawn E Quelle,William Skarnes,Jyoti S Choudhary

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHF16 抗体

Anti-PHF16 antibody

Immunoprecipitation - Anti-PHF16 antibody (AB129495)

Western blot - Anti-PHF16 antibody (AB129495)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise