Anti-PHF10 抗体 (ab154637)
Key features and details
- Rabbit polyclonal to PHF10
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PHF10 antibody
PHF10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PHF10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 243-459 of Human PHF10 (UniProt ID: Q8WUB8).
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ポジティブ･コントロール
- WB: HEK-293T and HeLa whole cell lysates; Mouse colon lysate. ICC: HeLa cells. IHC-P: HeLa xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154637の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
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IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
配列類似性
Belongs to the SAYP family.
Contains 2 PHD-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55274 Human
- Entrez Gene: 72057 Mouse
- Omim: 613069 Human
- SwissProt: Q8WUB8 Human
- SwissProt: Q9D8M7 Mouse
- Unigene: 435933 Human
- Unigene: 440085 Mouse
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別名
- BAF45a antibody
- BRG1-associated factor 45a antibody
- FLJ10975 antibody
see all
画像
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Western blot - Anti-PHF10 antibody (ab154637)All lanes : Anti-PHF10 antibody (ab154637) at 1/500 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : PHF10 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HeLa (Nuclear prepped) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa (possible non-specific binding)Lanes 1 - 4: Merged signal (red and green). Green - ab154637 observed at 60 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab154637 was shown to react with PHF10 in wild-type HEK-293 cells in Western blot with loss of signal observed in PHF10 knockout sample. Wild-type HEK-293 and PHF10 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154637 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-PHF10 antibody (ab154637)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PHF10 with ab154637 at a 1/500 dilution. Alpha-tubulin filaments were labeled (red) at 1/2000. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF10 antibody (ab154637)Immunohistochemical analysis of paraffin embedded Hela xenograft labeling PHF10 with ab154637 at a 1/500 dilution. -
Western blot - Anti-PHF10 antibody (ab154637)Anti-PHF10 antibody (ab154637) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 56 kDa
10% SDS PAGE -
Western blot - Anti-PHF10 antibody (ab154637)Anti-PHF10 antibody (ab154637) at 1/1000 dilution + mouse colon lysate/ extract at 50 µg
Predicted band size: 56 kDa
10 % SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154637 は 3 報の論文で使用されています。
- Wang Y et al. Circ_0001023 Promotes Proliferation and Metastasis of Gastric Cancer Cells Thro?ugh ?miR-409-3p/PHF10 Axis. Onco Targets Ther 13:4533-4544 (2020). PubMed: 32547084
- Fernando TM et al. Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patients. Nat Commun 11:5551 (2020). PubMed: 33144586
- Anbunathan H et al. Integrative Copy Number Analysis of Uveal Melanoma Reveals Novel Candidate Genes Involved in Tumorigenesis Including a Tumor Suppressor Role for PHF10/BAF45a. Clin Cancer Res 25:5156-5166 (2019). PubMed: 31227497