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Anti-PHD1/prolyl hydroxylase 抗体 [PHD112/G7] (ab82884)

Submit a review Q&A (1)References (1)

Key features and details

  • Mouse monoclonal [PHD112/G7] to PHD1/prolyl hydroxylase
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-PHD1/prolyl hydroxylase antibody [PHD112/G7]
    PHD1/prolyl hydroxylase 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [PHD112/G7] to PHD1/prolyl hydroxylase

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length Human protein

  • ポジティブ･コントロール

    • Human testis tissue

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab82884の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.

  • 組織特異性

    Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.

  • 配列類似性

    Contains 1 Fe2OG dioxygenase domain.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DKFZp434E026 antibody
      • EGL nine (C.elegans) homolog 2 antibody
      • Egl nine homolog 2 (C. elegans) antibody
      • Egl nine homolog 2 antibody
      • EGLN 2 antibody
      • EGLN2 antibody
      • EGLN2_HUMAN antibody
      • EIT 6 antibody
      • EIT6 antibody
      • Estrogen-induced tag 6 antibody
      • HIF P4H 1 antibody
      • HIF PH1 antibody
      • HIF prolyl hydroxylase 1 antibody
      • HIF-PH1 antibody
      • HIF-prolyl hydroxylase 1 antibody
      • HIFPH 1 antibody
      • HIFPH1 antibody
      • HPH 3 antibody
      • HPH-1 antibody
      • HPH-3 antibody
      • HPH3 antibody
      • Hypoxia inducible factor prolyl hydroxylase 1 antibody
      • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
      • P4H1 antibody
      • PHD 1 antibody
      • PhD1 antibody
      • prolyl hydroxylase domain containing protein 1 antibody
      • Prolyl hydroxylase domain-containing protein 1 antibody
      see all

    参考文献 (1)

    ab82884 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab82884 は 1 報の論文で使用されています。

    • Andersen S  et al. Overexpression of the HIF Hydroxylases PHD1, PHD2, PHD3 and FIH Are Individually and Collectively Unfavorable Prognosticators for NSCLC Survival. PLoS One 6:e23847 (2011). IHC-P ; Human . PubMed: 21887331

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    Your data sheet states this needs antigen retrieval- this is not correct as the originator of this mAB I have validated it for IHC and it will not work if you use antigen retrieval
    Best wishes

    Read More
    Answer

    Thank you for your enquiry regarding ab82884.

    This particular antibody has not been tested for IHC-P in the lab as standard procedure, however based on this specific publication it is suitable for this application. I would advise you to take a look at this published work for further information:

    Andersen S et al. Overexpression of the HIF Hydroxylases PHD1, PHD2, PHD3 and FIH Are Individually and Collectively Unfavorable Prognosticators for NSCLC Survival. PLoS One 6:e23847 (2011). IHC-P; Human.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

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