AB240176

Anti-PHD finger protein 6/PHF6 抗体 [EPR11997] - BSA and Azide free

Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal PHD finger protein 6/PHF6 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

別名を表示する

CENP-31, KIAA1823, PHF6, PHD finger protein 6, PHD-like zinc finger protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free (AB240176)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free (AB240176)

Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab173304 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab173304).

Supplier Data

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free (AB240176)

All lanes:

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (<a href='/products/primary-antibodies/phd-finger-protein-6-phf6-antibody-epr11997-ab173304'>ab173304</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Predicted band size: 41 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR11997

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

ab240176 is the carrier-free version of ab173304.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PHD finger protein 6 also known as PHF6 is a protein with a molecular mass of approximately 43 kDa. It consists of two PHD domains which are zinc finger-like motifs playing roles in chromatin remodeling. PHF6 expresses widely in various tissues with noticeable levels in the brain and hematopoietic cells indicating its significance in these areas. Alternate names for PHF6 include ZNF312 and BORJ although PHF6 is the most commonly referred designation.

Biological function summary

PHF6 involves a range of cellular processes including regulation of transcription and chromatin structure modification. PHF6 can bind to certain DNA sequences and proteins to influence gene expression. It interacts with chromatin remodeling complexes indicating a role in maintaining chromatin accessibility for transcription factors. The protein's PHD domains contribute to its ability to engage in these interactions impacting the transcriptional activity of target genes.

Pathways

PHF6 interacts with transcriptional and chromatin remodeling pathways. It plays a role in the interplay between chromatin and transcriptional machinery which is critical for regulating gene expression. PHF6 associates with proteins such as nucleophosmin and other chromatin regulators influencing pathways like the Notch signaling pathway which is vital for cellular differentiation and development. These interactions highlight its involvement in genetic regulation within developmental pathways.

Mutations in PHF6 link to Börjeson-Forssman-Lehmann syndrome (BFLS) a rare genetic disorder affecting neurological development. Additionally PHF6 mutations recurrently appear in T-cell acute lymphoblastic leukemia (T-ALL) highlighting a role in hematological malignancies. In the context of T-ALL PHF6 interacts with proteins like Notch1 which drives leukemic proliferation when dysregulated. These associations make PHF6 a significant focus for understanding and potentially targeting specific genetic disorders and cancers.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional regulator that associates with ribosomal RNA promoters and suppresses ribosomal RNA (rRNA) transcription.

See full target information PHF6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:860898 PubMed35401227

2022

Perftoran Inhibits Hypoxia-Associated Resistance in Lung Cancer Cells to Carboplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Amira M Gamal-Eldeen,Amani A Alrehaili,Afaf Alharthi,Bassem M Raafat

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHD finger protein 6/PHF6 抗体 [EPR11997] - BSA and Azide free

Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free (AB240176)

Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] - BSA and Azide free (AB240176)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise