Anti-PHD finger protein 1/PHF1 抗体 [EPR14222] (ab184951)

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Western blot - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14222] to PHF1
Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IP
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品名

Anti-PHD finger protein 1/PHF1 antibody [EPR14222]
PHD finger protein 1/PHF1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR14222] to PHF1
由来種

Rabbit
特異性

This antibody is specific to PHD finger protein 1, not paired helical filaments.
アプリケーション

適用あり: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IPmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- 293, Jurkat, MCF7 and Human fetal brain lysates; Human pancreas and Rat skeletal muscle tissues; MCF7 cells; 293 cells. Mouse and rat brain tissue lysate. NIH/3T3, RAW264.7, C6 and PC-12 whole cell lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR14222
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-PHD finger protein 1/PHF1 antibody [EPR14222] - BSA and Azide free (ab232601)
- Alexa Fluor® 568 Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab312482)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab184951の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.
WB		1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP		1/30.

特記事項
Flow Cyt (Intra) 1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP 1/30.

機能

Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
組織特異性

Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
配列類似性

Contains 2 PHD-type zinc fingers.
細胞内局在

Nucleus. Localizes specifically to the promoters of numerous target genes.
Target information above from: UniProt accession O43189 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 5252 Human
- Entrez Gene: 21652 Mouse
- Entrez Gene: 294287 Rat
- Omim: 602881 Human
- SwissProt: O43189 Human
- SwissProt: Q9Z1B8 Mouse
- Unigene: 166204 Human
- Unigene: 480590 Mouse
別名
- hPCl1 antibody
- MTF2 L2 antibody
- MTF2L 2 antibody
- MTF2L2 antibody
- PCL 1 antibody
- PCL1 antibody
- PHD finger protein 1 antibody
- PHD finger protein1 antibody
- PHF 1 antibody
- PHF 2 antibody
- Phf1 antibody
- PHF1_HUMAN antibody
- PHF2 antibody
- Plc 1 antibody
- Plc1 antibody
- Polycomb like protein 1 antibody
- Polycomb like protein1 antibody
- Polycomb-like protein 1 antibody
- Protein PHF1 antibody
- T complex testis expressed 3 antibody
- T complex testis expressed3 antibody
- Tctex 3 antibody
- Tctex3 antibody
see all

Western blot - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

All lanes : Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951) at 1/1000 dilution

Lane 1 : Mouse brain tissue
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 62 kDa
Immunoprecipitation - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

PHF1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab184951 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab184951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse brain tissue lysate 10 ug

Lane 2: ab184951 IP in Mouse brain tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184951 in mouse brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes
Western blot - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

All lanes : Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951) at 1/20000 dilution

Lane 1 : 293 lysate
Lane 2 : Jurkat lysate
Lane 3 : MCF7 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa
Western blot - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951) at 1/5000 dilution + Human fetal brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Flow Cytometry (Intracellular) - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

Intracellular flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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ab184951 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184951 は 10 報の論文で使用されています。

Yan F et al. Icariin ameliorates memory deficits through regulating brain insulin signaling and glucose transporters in 3×Tg-AD mice. Neural Regen Res 18:183-188 (2023). PubMed: 35799540
Qu M et al. High glucose induces tau hyperphosphorylation in hippocampal neurons via inhibition of ALKBH5-mediated Dgkh m6A demethylation: a potential mechanism for diabetic cognitive dysfunction. Cell Death Dis 14:385 (2023). PubMed: 37385994
Huang S et al. Chimeric cerebral organoids reveal the essentials of neuronal and astrocytic APOE4 for Alzheimer's tau pathology. Signal Transduct Target Ther 7:176 (2022). PubMed: 35691989
Lopez-Toledo G et al. Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis. Front Aging Neurosci 14:921573 (2022). PubMed: 35847683
Ning J et al. Down-regulated m6A reader FTO destabilizes PHF1 that triggers enhanced stemness capacity and tumor progression in lung adenocarcinoma. Cell Death Discov 8:354 (2022). PubMed: 35945194
Li YZ et al. Combined treatment with valproic acid and estrogen has neuroprotective effects in ovariectomized mice with Alzheimer's disease. Neural Regen Res 16:2078-2085 (2021). PubMed: 33642397
Alexandrova E et al. Interaction Proteomics Identifies ERbeta Association with Chromatin Repressive Complexes to Inhibit Cholesterol Biosynthesis and Exert An Oncosuppressive Role in Triple-negative Breast Cancer. Mol Cell Proteomics 19:245-260 (2020). PubMed: 31792072
Liu H et al. Guanosine monophosphate reductase 1 is a potential therapeutic target for Alzheimer's disease. Sci Rep 8:2759 (2018). PubMed: 29426890
Qu Y et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). PubMed: 30382790
Noël A et al. Sex-specific lung functional changes in adult mice exposed only to second-hand smoke in utero. Respir Res 18:104 (2017). WB ; Mouse . PubMed: 28651580

レビューと Q&A

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Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Testis)

Permeabilization

Yes - 0.1% Tween

Specification

Testis

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Mar 07 2018

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (10)

レビューと Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-PHF1 antibody [EPR14222]