Anti-PHAP1 抗体

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (AB5991)

Immunofluorescence of PHAP I in mouse small intestine cells using ab5991 at 20μg/ml.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAP1 antibody (AB5991)

ab5991 at 2μg/ml staining PHAP1 in mouse small intestine tissue by IHC

• WB

Supplier Data

Western blot - Anti-PHAP1 antibody (AB5991)

Incubated with the primary antibody for 1 hour at room temperature in 5% NFDM/TBST.

All lanes:

Western blot - Anti-PHAP1 antibody (ab5991) at 1 µg/mL

Lane 1:

293 cell lysate at 15 µg

Lane 2:

A431 cell lysate at 15 µg

Lane 3:

A549 cell lysate at 15 µg

Lane 4:

CaCo-2 cell lysate at 15 µg

Lane 5:

Daudi cell lysate at 15 µg

Lane 6:

HeLa cell lysate at 15 µg

Lane 7:

HepG2 cell lysate at 15 µg

Lane 8:

K562 cell lysate at 15 µg

Lane 9:

MCF-7 cell lysate at 15 µg

Lane 10:

Jurkat cell lysate at 15 µg

Lane 11:

SK-N-SH cell lysate at 15 µg

Lane 12:

THP-1 cell lysate at 15 µg

Lane 13:

NIH/3T3 cell lysate at 15 µg

Lane 14:

YB2/0 cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 28 kDa

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• WB

Supplier Data

Western blot - Anti-PHAP1 antibody (AB5991)

Incubated with the primary antibody for 1 hour at room temperature in 5% NFDM/TBST.

All lanes:

Western blot - Anti-PHAP1 antibody (ab5991) at 1 µg/mL

Lane 1:

Mouse heart tissue lysate at 15 µg/mL

Lane 2:

Mouse pancreas tissue lysate at 15 µg/mL

Lane 3:

Mouse testis tissue lysate at 15 µg/mL

Lane 4:

Mouse liver tissue lysate at 15 µg/mL

Lane 5:

Mouse spleen tissue lysate at 15 µg/mL

Lane 6:

Mouse kidney tissue lysate at 15 µg/mL

Lane 7:

Mouse colon tissue lysate at 15 µg/mL

Lane 8:

Mouse stomach tissue lysate at 15 µg/mL

Lane 9:

Mouse brain tissue lysate at 15 µg/mL

Lane 10:

Mouse skin tissue lysate at 15 µg/mL

Lane 11:

Mouse skeletal muscle tissue lysate at 15 µg/mL

Lane 12:

Mouse bladder tissue lysate at 15 µg/mL

Lane 13:

Mouse lung tissue lysate at 15 µg/mL

Lane 14:

K562 cell lysate at 15 µg/mL

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 28 kDa

false

• WB

Lab

Western blot - Anti-PHAP1 antibody (AB5991)

False colour image of Western blot : Anti-PHAP1 antibody staining at 0.5 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab5991 was shown to bind specifically to PHAP1. A band was observed at 33 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in ANP32A knockout cell line ab266148 (knockout cell lysate ab258303). To generate this image, wild-type and ANP32A knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Lanes 1 - 4:

Western blot - Anti-PHAP1 antibody (ab5991) at 0.5 µg/mL

Lanes 1 - 4:

Western blot - Anti-PHAP1 antibody (<a href='/products/primary-antibodies/phap1-antibody-ab5992'>ab5992</a>) at 0.5 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

ANP32A knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human ANP32A (PHAP1) knockout HEK-293T cell line (<a href='/products/cell-lines/human-anp32a-phap1-knockout-hek-293t-cell-line-ab266148'>ab266148</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Predicted band size: 28 kDa

Observed band size: 33 kDa

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• WB

Unknown

Western blot - Anti-PHAP1 antibody (AB5991)

Western blot analysis of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue llysates with anti-PHAP I at 1 μg/ml.

All lanes:

Western blot - Anti-PHAP1 antibody (ab5991)

Predicted band size: 28 kDa

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