Anti-PGRMC1 抗体 (ab48012)
Key features and details
- Goat polyclonal to PGRMC1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PGRMC1 antibody
PGRMC1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PGRMC1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig
-
免疫原
Synthetic peptide:
C-EPKDESARKND
, corresponding to C terminal amino acids 185-195 of Human PGRMC1. -
ポジティブ･コントロール
- WB: Human kidney, liver and testis lysates. Mouse testis.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48012の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.33 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
A 1 hour primary incubation at room temperature is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.33 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa). A 1 hour primary incubation at room temperature is recommended for this product. |
ターゲット情報
-
機能
Receptor for progesterone. -
組織特異性
Widely expressed, with highest expression in liver and kidney. -
配列類似性
Belongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain. -
ドメイン
The cytochrome b5 heme-binding domain lacks the conserved iron-binding His residues at positions 107 and 131. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 317706 Cow
- Entrez Gene: 10857 Human
- Entrez Gene: 53328 Mouse
- Entrez Gene: 396946 Pig
- Entrez Gene: 291948 Rat
- Omim: 300435 Human
- SwissProt: Q17QC0 Cow
- SwissProt: O00264 Human
see all -
別名
- HPR6.6 antibody
- Membrane associated progesterone receptor component 1 antibody
- Membrane-associated progesterone receptor component 1 antibody
see all
画像
-
Western blot - Anti-PGRMC1 antibody (ab48012)All lanes : Anti-PGRMC1 antibody (ab48012) at 0.1 µg/ml
Lane 1 : Human Kidney
Lane 2 : Human Liver
Lane 3 : Human Testes
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDa -
Western blot - Anti-PGRMC1 antibody (ab48012)Anti-PGRMC1 antibody (ab48012) at 0.1 µg/ml + Mouse Testes at 35 µg
Predicted band size: 22 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (15)
ab48012 は 15 報の論文で使用されています。
- Bai Y et al. PGRMC1 Promotes Progestin-Dependent Proliferation of Breast Cancer Cells by Binding Prohibitins Resulting in Activation of ERα Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 34830790
- Furuhata R et al. Progesterone receptor membrane associated component 1 enhances obesity progression in mice by facilitating lipid accumulation in adipocytes. Commun Biol 3:479 (2020). PubMed: 32887925
- Salsano S et al. Novel nonclassic progesterone receptor PGRMC1 pulldown-precipitated proteins reveal a key role during human decidualization. Fertil Steril 113:1050-1066.e7 (2020). PubMed: 32386616
- He Y et al. Combined assessment of low PGRMC1/positive ATP1A1 levels has enhanced prognostic value for renal cell carcinoma. Oncol Rep 40:1467-1476 (2018). PubMed: 30015972
- Riad A et al. Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex. Sci Rep 8:16845 (2018). PubMed: 30443021