AB242104

Anti-PGP 抗体

Name: Anti-PGP 抗体 (ab242104) | Abcam
Brand: Abcam Limited
SKU: AB242104
Price: 74000 JPY
Availability: InStock

Anti-PGP antibody

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal PGP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PGP aa 250 to C-terminus.

別名を表示する

Glycerol-3-phosphate phosphatase, G3PP, Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, Phosphoglycolate phosphatase, AUM, PGP

2 Images

Supplier Data

Immunoprecipitation - Anti-PGP antibody (AB242104)

PGP IP in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242104 at 8 μL per reaction. Western blot was performed on the immunoprecipitate using ab242104 at 1/400 dilution.

Lane 1 : ab242104 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-PGP antibody (ab242104)

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-PGP antibody (AB242104)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-PGP antibody (ab242104) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 34 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human PGP aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

A6NDG6

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "8 - 15 μl/mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antibody was affinity purified using an epitope specific to PGP immobilized on solid support.

バッファー組成

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PGP also known as P-glycoprotein or Pgp protein functions as an important membrane-associated ATP-dependent efflux transporter. It has a molecular weight of approximately 170 kDa. This protein is widely expressed in cells throughout the body with high levels present in the liver kidney intestine and blood-brain barrier. PGP's mechanical role involves transporting various substrates including drugs metabolic products and xenobiotics across cellular membranes which is important for protecting tissues from toxins.

Biological function summary

PGP plays an important role in the body's defense by contributing to the cellular excretion of potentially harmful substances. It is part of the ATP-binding cassette (ABC) transporters family. This protein helps maintain cellular homeostasis by regulating the intracellular concentration of substrates. PGP products influence pharmacokinetics by affecting drug absorption and excretion which can affect therapy outcomes. The study of PGP alternatives may provide insights into overcoming drug resistance in various conditions.

Pathways

PGP participates in two major biological systems: the metabolic pathway and the detoxification pathway. In these pathways PGP works closely with other proteins such as cytochrome P450 enzymes to metabolize and eliminate substances from cells. Together with multidrug resistance-associated proteins PGP contributes to the intricate network of cellular defense mechanisms. The proper functioning of these pathways is essential for protecting tissues from damage due to toxins and drug accumulation.

PGP's activity is significant in influencing the development of multidrug resistance in cancer treatment and the progression of neurodegenerative diseases like Alzheimer's disease. Altered PGP expression can lead to inefficient drug clearance in cancer making chemotherapy less effective and allowing cancer cells to survive. In Alzheimer's disease overexpression may reduce the clearance of amyloid-beta a protein linked to the condition which leads to its accumulation in the brain. Consequently research into modulating PGP expression and function is critical for improving therapeutic strategies in these disorders.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed : 26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).

See full target information PGP

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:9070-9085 PubMed35293280

2022

Circular RNA circ_0002360 regulates the Taxol resistance and malignant behaviors of Taxol-resistant non-small cell lung cancer cells by microRNA-585-3p-dependent modulation of G protein regulated inducer of neurite outgrowth 1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohai Cui,Boxiang Zhang,Baocheng Li,Xinju Li

Molecular medicine reports 24: PubMed34036375

2021

lncRNA TUG1 inhibits the cancer stem cell‑like properties of temozolomide‑resistant glioma cells by interacting with EZH2.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiang Cao,Wenying Chai,Yonggang Wang,Dang Tang,Dongchuan Shao,Hai Song,Jiang Long

Molecular medicine reports 23: PubMed33760140

2021

Inhibition of chaperone‑mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xuan,Shuang Zhao,Xingjun Xiao,Liwei Xiang,Hua-Chuan Zheng

Cancer management and research 12:10055-10065 PubMed33116862

2020

Knockdown of circ-TTBK2 Inhibits Glioma Progression by Regulating miR-1283 and CHD1.

Applications

Unspecified application

Species

Unspecified reactive species

Chengchen Han,Shuwei Wang,Hongwei Wang,Jianning Zhang

Experimental and therapeutic medicine 18:2731-2738 PubMed31572520

2019

Reduced pim-1 expression increases chemotherapeutic drug sensitivity in human androgen-independent prostate cancer cells by inducing apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Zhang,Yuyan Sun,Peng Wang,Changfu Yang,Shengwei Li

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PGP 抗体

Anti-PGP antibody

Immunoprecipitation - Anti-PGP antibody (AB242104)

Western blot - Anti-PGP antibody (AB242104)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise