Anti-PGK1 抗体 [22C5D8] (ab113687)
![Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)](https://www.abcam.co.jp/ps/products/113/ab113687/Images/ab113687-126381-anti-pgk1-antibody-22c5d8-western-blot.jpg "Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)")
Key features and details
- Mouse monoclonal [22C5D8] to PGK1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-PGK1 antibody [22C5D8]
PGK1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [22C5D8] to PGK1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
22C5D8 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113687の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
ターゲット情報
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機能
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). -
パスウェイ
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. -
関連疾患
Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations. -
配列類似性
Belongs to the phosphoglycerate kinase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 850370 Saccharomyces cerevisiae
- SwissProt: P00560 Saccharomyces cerevisiae
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別名
- Cell migration-inducing gene 10 protein antibody
- Epididymis secretory sperm binding protein Li 68p antibody
- HEL S 68p antibody
see all
画像
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Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)Anti-PGK1 antibody [22C5D8] (ab113687) + Yeast whole cell lysate at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa -
![Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)](https://www.abcam.co.jp/ps/products/113/ab113687/Images/ab113687-343896-anti-pgk1-antibody-22c5d8-western-blot-Saccharomyces-cerevisiae.jpg)
Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)This image is courtesy of an anonymous abreview.Anti-PGK1 antibody [22C5D8] (ab113687) at 1/1000 dilution (16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk/ TBS-T) + Saccharomyces cerevisiae Cell lysate - nuclear at 30 µg
Secondary
Sheep anti-Mouse IgG, HRP-linked whole Ab ECL at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 1 minuteMilk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C.
Gel running condition: Reduced Denaturing (4-12% Bis-Tris).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (143)
ab113687 は 143 報の論文で使用されています。
- Luo J et al. Acinetobacter baumannii Kills Fungi via a Type VI DNase Effector. mBio 14:e0342022 (2023). PubMed: 36625573
- Barnett SE et al. BAP1 Loss Is Associated with Higher ASS1 Expression in Epithelioid Mesothelioma: Implications for Therapeutic Stratification. Mol Cancer Res 21:411-427 (2023). PubMed: 36669126
- Onishi M et al. The GET pathway serves to activate Atg32-mediated mitophagy by ER targeting of the Ppg1-Far complex. Life Sci Alliance 6:N/A (2023). PubMed: 36697253
- Wang M et al. L3MBTL3 is induced by HIF-1α and fine tunes the HIF-1α degradation under hypoxia in vitro. Heliyon 9:e13222 (2023). PubMed: 36747531
- Chau S et al. Diverse yeast antiviral systems prevent lethal pathogenesis caused by the L-A mycovirus. Proc Natl Acad Sci U S A 120:e2208695120 (2023). PubMed: 36888656