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リコンビナントRabMAb

Anti-PGK1 + PGK2 抗体 [EPR14908] (ab186742)

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Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
  • Flow Cytometry (Intracellular) - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
  • Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14908] to PGK1 + PGK2
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PGK1 + PGK2 antibody [EPR14908]
    PGK1 + PGK2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14908] to PGK1 + PGK2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human testis lysate; Jurkat, HepG2, HeLa, C6, Raw 264.7, PC-12 and NIH 3T3 cell lysates; Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186742の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
特記事項
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

画像

  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    All lanes : Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/5000 dilution

    Lane 1 : PGK1 Human Recombinant Protein
    Lane 2 : PGK2 Human Recombinant Protein

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa


    Exposure time: 60 seconds


    Blocking and Diluting Buffer: 5% NFDM/TBST

  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    All lanes : Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Human testis cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    Western blot - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    All lanes : Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Flow Cytometry (Intracellular) - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    Flow Cytometry (Intracellular) - Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells labeling PGK1 with ab186742 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

     

     

  • Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)
    Anti-PGK1 + PGK2 antibody [EPR14908] (ab186742)

参考文献 (2)

ab186742 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186742 は 2 報の論文で使用されています。

  • Zhang C  et al. ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma. J Cell Mol Med 26:5078-5094 (2022). PubMed: 36071546
  • Liu XX  et al. Screening targeted testis-specific genes for molecular assessment of aberrant sperm quality. Mol Med Rep 14:1594-600 (2016). PubMed: 27356588

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