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AB217966

Anti-PGE2 receptor EP4 subtype 抗体

Anti-PGE2 receptor EP4 subtype antibody

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(5 Publications)

Rabbit Polyclonal PGE2 receptor EP4 subtype antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PTGER4 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

PTGER2, PTGER4, Prostaglandin E2 receptor EP4 subtype, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB217966)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of human lung carcinoma tissue labeling PGE2 receptor EP4 with ab217966 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

Western blot - Anti-PGE2 receptor EP4 subtype antibody (AB217966)
  • WB

Lab

Western blot - Anti-PGE2 receptor EP4 subtype antibody (AB217966)

All lanes:

Western blot - Anti-PGE2 receptor EP4 subtype antibody (ab217966) at 1/300 dilution

All lanes:

Raji cell lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/20000 dilution

Predicted band size: 53 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human PTGER4 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35408

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The PGE2 receptor EP4 subtype also known as PTGER4 is a member of the G protein-coupled receptor (GPCR) family. This receptor binds prostaglandin E2 and plays a role in various physiological processes. The protein has an approximate mass of 65 kDa. EP4 receptor is widely expressed in tissues such as the immune system bone gastrointestinal tract and reproductive organs indicating its diverse functional implications. Additionally EP4 receptor is activated by PGE2 EP4 agonists which help in modulating its activity.
Biological function summary

EP4 receptor influences numerous functions including inflammation immune response and bone metabolism. The receptor is not part of a larger complex but interacts with different signaling molecules and secondary messengers during its activity. It modulates responses mainly through cAMP which further amplifies or reduces cellular responses depending on the context. These biological roles display the versatile effects EP4 can exert during various physiological scenarios.

Pathways

The EP4 receptor is a significant component in the prostaglandin signaling pathway. This pathway involves the conversion of arachidonic acid to prostaglandin E2 followed by binding to EP4 triggering downstream effects via cAMP-related signals. Within this pathway it interacts closely with proteins like adenylate cyclase which plays a role in regulating cAMP levels. EP4 also participates in the Wnt signaling pathway where it can either enhance or inhibit processes by interacting with other signaling cascade proteins.

EP4 receptor has connections with conditions like rheumatoid arthritis and bone-related diseases. In rheumatoid arthritis excessive signaling through EP4 exacerbates inflammatory responses in joint tissue. The receptor potentially associates with inflammatory cytokines like tumor necrosis factor-alpha (TNF-alpha) contributing to disease severity. In bone-related diseases such as osteoporosis EP4 influences bone remodeling and homeostasis linking to proteins involved in bone formation and resorption dynamics.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
See full target information PTGER4
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40028769

2025

The Kv2.2 channel mediates the inhibition of prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chengfang Pan,Ying Liu,Liangya Wang,Wen-Yong Fan,Yunzhi Ni,Xuefeng Zhang,Di Wu,Chenyang Li,Jin Li,Zhaoyang Li,Rui Liu,Changlong Hu

Cellular and molecular life sciences : CMLS 79:303 PubMed35588334

2022

Macrophages evoke autophagy of hepatic stellate cells to promote liver fibrosis in NAFLD mice via the PGE2/EP4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Cao,Weili Mai,Rui Li,Shuwei Deng,Lan Li,Yanxi Zhou,Qiushi Qin,Yue Zhang,Xingang Zhou,Ming Han,Pu Liang,Yonghong Yan,Yu Hao,Wen Xie,Jie Yan,Liuluan Zhu

Cell reports 36:109674 PubMed34496236

2021

Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche.

Applications

Unspecified application

Species

Unspecified reactive species

Xi He,Sarah E Smith,Shiyuan Chen,Hua Li,Di Wu,Paloma I Meneses-Giles,Yongfu Wang,Mark Hembree,Kexi Yi,Xia Zhao,Fengli Guo,Jay R Unruh,Lucinda E Maddera,Zulin Yu,Allison Scott,Anoja Perera,Yan Wang,Chongbei Zhao,KyeongMin Bae,Andrew Box,Jeffrey S Haug,Fang Tao,Deqing Hu,Darrick M Hansen,Pengxu Qian,Subhrajit Saha,Dan Dixon,Shrikant Anant,Da Zhang,Edward H Lin,Weijing Sun,Leanne M Wiedemann,Linheng Li

Oxidative medicine and cellular longevity 2021:5529430 PubMed34326916

2021

Exosomal miR-218-5p/miR-363-3p from Endothelial Progenitor Cells Ameliorate Myocardial Infarction by Targeting the p53/JMY Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ke,Rongfeng Yang,Fang Wu,Xing Wang,Jiawen Liang,Xun Hu,Chengheng Hu

Cancers 13: PubMed33808776

2021

EP4 as a Negative Prognostic Factor in Patients with Vulvar Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Buchholz,Aurelia Vattai,Sophie Fürst,Theresa Vilsmaier,Christina Kuhn,Elisa Schmoeckel,Doris Mayr,Christian Dannecker,Sven Mahner,Udo Jeschke,Helene H Heidegger
View all publications
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代替製品

Primary Antibodies

AB188758

Anti-PGE2 receptor EP4 subtype antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody (AB188758)

  • 3
  • 2
Primary Antibodies

AB188761

Anti-PGE2 receptor EP4 subtype antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGE2 receptor EP4 subtype antibody - C-terminal (AB188761)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB167171

Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (AB167171)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB217925

Anti-Prostaglandin E Receptor EP1/PTGER1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Receptor EP1/PTGER1 antibody (AB217925)

  • 0
  • 0

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