Anti-PGC1 alpha + beta 抗体 - BSA and Azide free (ab54481)
Key features and details
- Rabbit polyclonal to PGC1 alpha + beta - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PGC1 alpha + beta antibody - BSA and Azide free
PGC1 alpha + beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PGC1 alpha + beta - BSA and Azide free -
由来種
Rabbit -
特異性
The immunogen sequence for this antibody was from PGC1 alpha, and is 78% homologous with PGC1 beta (Uniprot: Q86YN6), which this antibody also detects.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Chicken, Cow, Dog, Pig, Cynomolgus monkey, Rhesus monkey, Opossum
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免疫原
Synthetic peptide corresponding to Human PGC1 alpha + beta aa 750-850.
Database link: Q9UBK2 -
ポジティブ･コントロール
- WB: Human PGC1 alpha recombinant cell lysate, Human PGC1 beta recombinant cell lysate, Human skeletal muscle cell lysate, Human heart cell lysate, Human kidney cell lysate
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特記事項
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54481の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (21) |
1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 92 kDa).
Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low. |
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 92 kDa). Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low. |
ターゲット情報
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関連性
PGC1 alpha - The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene). PGC1 beta - Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. -
細胞内局在
Nucleus -
参照データベース
- Entrez Gene: 10891 Human
- Entrez Gene: 133522 Human
- Entrez Gene: 170826 Mouse
- Entrez Gene: 19017 Mouse
- Omim: 604517 Human
- Omim: 608886 Human
- SwissProt: Q86YN6 Human
- SwissProt: Q9UBK2 Human
see all -
別名
- LEM6 antibody
- Ligand effect modulator 6 antibody
- PERC antibody
see all
画像
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Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481)All lanes : Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481)
Lane 1 : Human PGC1 alpha recombinant (aa481-798) cell lysate at 0.1 µg
Lane 2 : Human PGC1 beta recombinant (aa550-1023 ) cell lysate at 0.1 µg
Lane 3 : Human skeletal muscle cell lysate at 20 µg
Lane 4 : Human heart cell lysate at 20 µg
Lane 5 : Human kidney cell lysate at 20 µg
Secondary
All lanes : ab216773 + ab216778 at 1/20000 dilution
Predicted band size: 92 kDaFalse colour image of Western blot: Anti-PGC1 alpha + beta antibody - BSA and Azide free staining at 1 µg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab54481 was shown to bind to PGC1 alpha and PGC1 beta. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.
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Western blot - Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481)All lanes : Anti-PGC1 alpha + beta antibody - BSA and Azide free (ab54481) at 1/2000 dilution
Lane 1 : Mouse Brown Adipose Tissue at 10 µg
Lane 2 : Mouse Brown Adipose Tissue at 20 µg
Predicted band size: 92 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?Western blot using ab54481 at 1:2000 to detect PGC1 in Mouse brown adipose tissue whole cell extract (both lanes).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (548)
ab54481 は 548 報の論文で使用されています。
- Liu L et al. Sirtuin 3 relieves inflammatory responses elicited by lipopolysaccharide via the PGC1α-NFκB pathway in bovine mammary epithelial cells. J Dairy Sci 106:1315-1329 (2023). PubMed: 36494223
- Li D et al. PGC-1α Affects Epileptic Seizures by Regulating Mitochondrial Fusion in Epileptic Rats. Neurochem Res 48:1361-1369 (2023). PubMed: 36454394
- Basten AM et al. Early initiation of electrical stimulation paired with range of motion after a volumetric muscle loss injury does not benefit muscle function. Exp Physiol 108:76-89 (2023). PubMed: 36116106
- Peng K et al. Rosmarinic acid protects against lipopolysaccharide-induced cardiac dysfunction via activating Sirt1/PGC-1α pathway to alleviate mitochondrial impairment. Clin Exp Pharmacol Physiol 50:218-227 (2023). PubMed: 36350269
- Maniscalco E et al. Metformin regulates myoblast differentiation through an AMPK-dependent mechanism. PLoS One 18:e0281718 (2023). PubMed: 36763621
