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遺伝子ノックアウト細胞株 検証済

Anti-PGAM5 抗体 [CL0624] (ab244218)

Submit a review Submit a question References (3)
Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
  • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)

Key features and details

  • Mouse monoclonal [CL0624] to PGAM5
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-PGAM5 antibody [CL0624]
    PGAM5 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CL0624] to PGAM5

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment corresponding to Human PGAM5 aa 50-150.
    Database link: Q96HS1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • エピトープ

    Binds to an epitope located within the peptidesequence NWDRREPLSLINVRK as determined by overlapping synthetic peptides.

  • ポジティブ･コントロール

    • IHC-P: Human kidney, liver, testis, uterus, tonsil and small intestine tissues. WB: HeLa, HEPG2, Daudi and RT4 cell lysates.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    CL0624

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab244218の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
特記事項
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.

ターゲット情報

  • 機能

    Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression.

  • 配列類似性

    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

  • ドメイン

    According to PubMed:18387606, may contain a non-cleaved N-terminal mitochondrial targeting sequence that targets PGAM5 to the cytosolic side of the outer mitochondrial membrane, instead of a N-terminal transmembrane.

  • 細胞内局在

    Mitochondrion. Mitochondrion outer membrane. Membrane. Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Bcl-XL-binding protein v68 antibody
      • BXLBv68 antibody
      • MGC5352 antibody
      • mitochondrial antibody
      • PGAM5 antibody
      • PGAM5_HUMAN antibody
      • Phosphoglycerate mutase family member 5 antibody
      • Serine/threonine protein phosphatase PGAM5 mitochondrial antibody
      • Serine/threonine-protein phosphatase PGAM5 antibody
      see all

    画像

    • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
      Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
      All lanes : Anti-PGAM5 antibody [CL0624] (ab244218)

      Lane 1 : Wild-type HeLa lysate
      Lane 2 : PGAM5 knockout HeLa lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Daudi lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 32 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab244218 observed at 32 kDa. Red - loading control ab52866 observed at 50 kDa.

       ab244218 Anti-PGAM5 antibody [CL0624] was shown to specifically react with PGAM5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265141 (knockout cell lysate ab257581) was used. Wild-type and PGAM5 knockout samples were subjected to SDS-PAGE. ab244218 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

      Paraffin-embedded human small intestine tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

      Paraffin-embedded human tonsil tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

      Paraffin-embedded human uterus tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

      Paraffin-embedded human testis tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

      Paraffin-embedded human liver tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM5 antibody [CL0624] (ab244218)

      Paraffin-embedded human kidney tissue stained for PGAM5 using ab244218 at 1/200 dilution in immunohistochemical analysis.

    • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
      Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
      All lanes : Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg/ml

      Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate with siRNA control
      Lane 2 : RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 1
      Lane 3 : RT4 (human urinary bladder cancer cell line) cell lysate with siRNA probe 2

      Predicted band size: 32 kDa

    • Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
      Western blot - Anti-PGAM5 antibody [CL0624] (ab244218)
      Anti-PGAM5 antibody [CL0624] (ab244218) at 1 µg + RT4 (human urinary bladder cancer cell line) cell lysate

      Predicted band size: 32 kDa

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (3)

    ab244218 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab244218 は 3 報の論文で使用されています。

    • Chatterjee A  et al. MnTE-2-PyP protects fibroblast mitochondria from hyperglycemia and radiation exposure. Redox Biol 52:102301 (2022). PubMed: 35358851
    • Xia R  et al. Moxibustion alleviates chronic heart failure by regulating mitochondrial dynamics and inhibiting autophagy. Exp Ther Med 23:359 (2022). PubMed: 35493422
    • Horvath C  et al. Inhibition of Cardiac RIP3 Mitigates Early Reperfusion Injury and Calcium-Induced Mitochondrial Swelling without Altering Necroptotic Signalling. Int J Mol Sci 22:N/A (2021). PubMed: 34360749

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