Anti-PFKP 抗体 (ab186132)
Key features and details
- Rabbit polyclonal to PFKP
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PFKP antibody
PFKP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PFKP -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide within Human PFKP aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_002618.1).
Database link: Q01813 -
ポジティブ･コントロール
- HeLa, 293T, Jurkat and NIH 3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab186132 was affinity purified using an epitope specific to PFKP immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186132の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 86 kDa. |
ターゲット情報
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パスウェイ
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. -
配列類似性
Belongs to the phosphofructokinase family. Two domains subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 450266 Chimpanzee
- Entrez Gene: 5214 Human
- Entrez Gene: 56421 Mouse
- Entrez Gene: 100173619 Orangutan
- Entrez Gene: 722330 Rhesus monkey
- Omim: 171840 Human
- SwissProt: Q01813 Human
- SwissProt: Q9WUA3 Mouse
see all -
別名
- 1200015H23Rik antibody
- 6 phosphofructokinase type C antibody
- 6 phosphofructokinase, platelet type antibody
see all
画像
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Western blot - Anti-PFKP antibody (ab186132)All lanes : Anti-PFKP antibody (ab186132) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PFKP antibody (ab186132)Detection of PFKP in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186132 at 6 µg/reaction for IP. For WB detection, ab186132 was used at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186132 は 1 報の論文で使用されています。
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). PubMed: 28607489