Anti-PFKM 抗体 [EPR10734(B)] (ab154804)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PFKM with purified ab154804 at 1/300 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling PFKM with Purified ab154804 at 1:300 dilution (8.7μg/ml). Cells were fixed in 100% Methanol and cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot analysis of Pig skeletal muscle tissue lysate using ab154804 (1:2000 in 1x PBS + 0.1% Tween-20 for 1 hour at 22°C) to label PFKM. A IRDye® 680-conjugated Donkey anti-rabbit IgG (1:40000) was used as the secondary antibody. The image shows increasing amounts of loaded protein for antibody optimization. The far left lane is the molecular weight standard.

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Immunofluorescence analysis of HeLa cells labeling PFKM with ab154804 at 1/50 dilution.

Intracellular flow cytometric analysis of permeabilized SHSY5Y cells labeling PFKM with ab154804 at 1/100 dilution (red) or a rabbit IgG (green - negative control).