Anti-PFKM + PFKL 抗体 [EPR11904] (ab181064)
![Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)](https://www.abcam.co.jp/ps/products/181/ab181064/Images/ab181064-472940-anti-pfkl-pfkm-antibody-western-blot-recombinant-protein.png "Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11904] to PFKM + PFKL
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PFKM + PFKL antibody [EPR11904] -
製品の詳細
Rabbit monoclonal [EPR11904] to PFKM + PFKL -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HepG2, A431, HeLa, C6, Raw264.7, PC-12 and NIH-3T3 cell lysates; ICC/IF: HeLa Flow Cyt (intra): C6 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11904 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181064の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 85 kDa).
|
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| ICC/IF |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 85 kDa). |
|
ICC/IF
1/50 - 1/100. |
ターゲット情報
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細胞内局在
PFKL: Cytoplasmic -
参照データベース
- Entrez Gene: 5211 Human
- Entrez Gene: 5213 Human
- Entrez Gene: 18641 Mouse
- Entrez Gene: 18642 Mouse
- Entrez Gene: 25741 Rat
- Entrez Gene: 65152 Rat
- Omim: 171860 Human
- Omim: 610681 Human
see all
画像
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Western blot - Anti-PFKM + PFKL antibody [EPR11904] (ab181064)All lanes : Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/1000 dilution
Lane 1 : GST tagged Recombinant Human PFKL protein (Full length, 116 KDa) with NFDM/TBST
Lane 2 : DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa) with NFDM/TBST
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated)
Predicted band size: 85 kDa
Observed band size: 116,58 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds -
![Western blot - Anti-PFKL antibody [EPR11904] (ab181064)](https://www.abcam.co.jp/ps/products/181/ab181064/Images/ab181064-213238-anti-pfkl-antibody-epr11904-western-blot.jpg)
Western blot - Anti-PFKL antibody [EPR11904] (ab181064)All lanes : Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 85 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-PFKL antibody [EPR11904] (ab181064)](https://www.abcam.co.jp/ps/products/181/ab181064/Images/ab181064-213237-anti-pfkl-antibody-epr11904-western-blot.jpg)
Western blot - Anti-PFKL antibody [EPR11904] (ab181064)All lanes : Anti-PFKM + PFKL antibody [EPR11904] (ab181064) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution
Predicted band size: 85 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody [EPR11904] (ab181064)](https://www.abcam.co.jp/ps/products/181/ab181064/Images/ab181064-213236-anti-pfkl-antibody-epr11904-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PFKL antibody [EPR11904] (ab181064)Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) staining PFKL with ab181064 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
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![Flow Cytometry (Intracellular) - Anti-PFKL antibody [EPR11904] (ab181064)](https://www.abcam.co.jp/ps/products/181/ab181064/Images/ab181064-213235-anti-pfkl-antibody-epr11904-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PFKL antibody [EPR11904] (ab181064)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedC6 cells labeling PFKL with ab181064 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Anti-PFKL antibody [EPR11904] (ab181064)](https://www.abcam.co.jp/ps/products/181/ab181064/Images/ab181064-5-benefits-of-recombinant-antibodies.png)
Anti-PFKL antibody [EPR11904] (ab181064)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (12)
ab181064 は 12 報の論文で使用されています。
- Tang HY et al. PDGFRβ modulates aerobic glycolysis in osteosarcoma HOS cells via the PI3K/AKT/mTOR/c-Myc pathway. Biochem Cell Biol 100:75-84 (2022). PubMed: 34678088
- Li JQ et al. Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro. Stem Cells Int 2022:4363632 (2022). PubMed: 35069747
- Hu B et al. IFNα Potentiates Anti-PD-1 Efficacy by Remodeling Glucose Metabolism in the Hepatocellular Carcinoma Microenvironment. Cancer Discov 12:1718-1741 (2022). PubMed: 35412588
- Pan Z et al. UBE2D3 Activates SHP-2 Ubiquitination to Promote Glycolysis and Proliferation of Glioma via Regulating STAT3 Signaling Pathway. Front Oncol 11:674286 (2021). PubMed: 34195079
- Chen L et al. Downregulation of GTSE1 leads to the inhibition of proliferation, migration, and Warburg effect in cervical cancer by blocking LHDA expression. J Obstet Gynaecol Res 47:3913-3922 (2021). PubMed: 34482592