AB318193

Anti-PFKFB4 抗体 [EPR28633-53]

Anti-PFKFB4 antibody [EPR28633-53]

KD Validated
20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal PFKFB4 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.

別名を表示する

PFK/FBPase 4, PFKFB4

3 Images

Supplier Data

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (AB318193)

Low expression : A549

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193) at 1/1000 dilution

Lane 1:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 50 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 54 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (AB318193)

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

HeLa transfected with siRNA specifically targeting PFKFB4 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 54 kDa,36 kDa

true

Exposure time: 81s

Supplier Data

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (AB318193)

This antibody does not cross-react with human PFKFB1, PFKFB2, PFKFB3.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193) at 1/1000 dilution

Lane 1:

293T cells transfected with a Human PFKFB1 expression vector containing a His-tag, whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a Human PFKFB2 expression vector containing a His-tag, whole cell lysate at 4 µg

Lane 3:

293T cells transfected with a Human PFKFB3 expression vector containing a His-tag, whole cell lysate at 4 µg

Lane 4:

293T cells transfected with a Human PFKFB4 expression vector containing a His-tag, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 54 kDa,36 kDa

false

Exposure time: 15s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28633-53

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PFKFB4 also known as 6-phosphofructo-2-kinase/fructose-26-bisphosphatase 4 is an enzyme with a molecular mass of approximately 66 kDa. It regulates the levels of fructose-26-bisphosphate by catalyzing both its synthesis and degradation. This enzyme predominantly exists in the cytoplasm and shows higher expression in tissues with fast cell growth and high glycolytic demand like the liver and various cancerous cells. PFKFB4 serves as an important regulatory node in cellular metabolism by controlling the flux of glucose through glycolysis.

Biological function summary

PFKFB4 influences metabolic reprogramming during cell proliferation and stress conditions. It is particularly important in the glycolytic shift known as the Warburg effect where cancer cells prefer glycolysis over oxidative phosphorylation leading to rapid glycolysis and lactic acid production even in the presence of oxygen. The enzyme is often part of a complex involving other glycolytic enzymes affecting both energy production and biosynthetic processes necessary for tumor growth and survival.

Pathways

PFKFB4 plays an integral role in the glycolytic and pentose phosphate pathways. It regulates glycolysis by modulating the concentration of fructose-26-bisphosphate which is an allosteric activator of phosphofructokinase-1 (PFK-1). PFKFB4 also interfaces with AMPK signaling which ties energy status of the cell to metabolic pathways further linking it to responses to energy stress and hypoxia in cancer cells.

PFKFB4 has strong associations with cancer and metabolic disorders. Overexpression of PFKFB4 is found in several cancer types including breast and prostate cancer where it supports anabolic processes and survival under metabolic stress. The enzyme closely relates to oncogenic proteins such as MYC which drives glycolytic gene expression and HIF-1α a factor that promotes glycolysis under hypoxia. Analyzing PFKFB4 provides insights into therapeutic approaches focused on targeting metabolic pathways in cancer.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Synthesis and degradation of fructose 2,6-bisphosphate.

See full target information PFKFB4

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFKFB4 抗体 [EPR28633-53]

Anti-PFKFB4 antibody [EPR28633-53]

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (AB318193)

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (AB318193)

Western blot - Anti-PFKFB4 antibody [EPR28633-53] (AB318193)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise