Anti-PFDN1 抗体 [EPR8547] (ab151708)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708)](https://www.abcam.co.jp/ps/products/151/ab151708/Images/ab151708-158741-anti-pfdn1-antibody-epr8547-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8547] to PFDN1
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PFDN1 antibody [EPR8547]
PFDN1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8547] to PFDN1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal brain, HepG2, HT1080, PC12 and NIH 3T3 lysates; Human hepatocellular carcinoma tissue; Human kidney tissue; PC12 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8547 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151708の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. -
配列類似性
Belongs to the prefoldin subunit beta family. - Information by UniProt
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参照データベース
- Entrez Gene: 5201 Human
- Entrez Gene: 67199 Mouse
- Entrez Gene: 361310 Rat
- Omim: 604897 Human
- SwissProt: O60925 Human
- SwissProt: Q9CWM4 Mouse
- Unigene: 483564 Human
- Unigene: 30184 Mouse
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別名
- PDF antibody
- PFD1 antibody
- PFD1_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PFDN1 with ab151708 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-PFDN1 antibody [EPR8547] (ab151708)](https://www.abcam.co.jp/ps/products/151/ab151708/Images/ab151708-158742-anti-pfdn1-antibody-epr8547-western-blot.jpg)
Western blot - Anti-PFDN1 antibody [EPR8547] (ab151708)All lanes : Anti-PFDN1 antibody [EPR8547] (ab151708) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HT1080 cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 14 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708)](https://www.abcam.co.jp/ps/products/151/ab151708/Images/ab151708-158740-anti-pfdn1-antibody-epr8547-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN1 antibody [EPR8547] (ab151708)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PFDN1 with ab151708 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] (ab151708)](https://www.abcam.co.jp/ps/products/151/ab151708/Images/ab151708-158739-anti-pfdn1-antibody-epr8547-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PFDN1 antibody [EPR8547] (ab151708)Intracellular flow cytometric analysis of permeabilized PC12 cells labeling PFDN1 with ab151708 at 1/100 dilution (red). Rabbit IgG negative control (green).
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![Anti-PFDN1 antibody [EPR8547] (ab151708)](https://www.abcam.co.jp/ps/products/151/ab151708/Images/ab151708-5-benefits-of-recombinant-antibodies.png)
Anti-PFDN1 antibody [EPR8547] (ab151708)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab151708 は 3 報の論文で使用されています。
- Gao Q et al. Functional Passenger-Strand miRNAs in Exosomes Derived from Human Colon Cancer Cells and Their Heterogeneous Paracrine Effects. Int J Biol Sci 16:1044-1058 (2020). PubMed: 32140072
- Peñate X et al. Overexpression of Canonical Prefoldin Associates with the Risk of Mortality and Metastasis in Non-Small Cell Lung Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32344577
- Chen X et al. Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules 22:N/A (2017). PubMed: 28858244