Anti-PFD6 抗体 (ab184984)
Key features and details
- Rabbit polyclonal to PFD6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PFD6 antibody -
製品の詳細
Rabbit polyclonal to PFD6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog
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免疫原
Recombinant fragment corresponding to Human PFD6 aa 50 to the C-terminus.
Database link: O15212 -
ポジティブ･コントロール
- IHC-P: Human colon, liver, pancreas and testis tissue; WB: RT-4 and U-251 MG sp whole cell lysate; ICC: U-2 OS whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
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機能
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. -
配列類似性
Belongs to the prefoldin subunit beta family. - Information by UniProt
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参照データベース
- Entrez Gene: 509914 Cow
- Entrez Gene: 474872 Dog
- Entrez Gene: 10471 Human
- Entrez Gene: 14976 Mouse
- Entrez Gene: 309629 Rat
- Omim: 605660 Human
- SwissProt: Q17Q89 Cow
- SwissProt: Q5TJE6 Dog
see all -
別名
- H2 KE2 antibody
- H2KE2 antibody
- HKE2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)
Immunohistochemistry analysis of human testis tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-PFD6 antibody (ab184984)All lanes : Anti-PFD6 antibody (ab184984) at 1/500 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) whole cell lysate
Predicted band size: 15 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (ab184984)Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling PFG6 at the nucleus and the Golgi apparatus with ab184984 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)Immunohistochemistry analysis of human pancreas tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)Immunohistochemistry analysis of human colon tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (ab184984)Immunohistochemistry analysis of human liver tissue labeling PFG6 with ab184984 at a 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab184984 は論文での使用が確認できていません。