AB184984

Anti-PFD6 抗体

Name: Anti-PFD6 抗体 (ab184984) | Abcam
Brand: Abcam Limited
SKU: AB184984
Price: 89000 JPY
Availability: InStock

Anti-PFD6 antibody

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal PFD6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PFDN6 aa 50 to C-terminus.

別名を表示する

HKE2, PFD6, PFDN6, Prefoldin subunit 6, Protein Ke2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human liver tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (AB184984)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (AB184984)

Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling PFG6 at the nucleus and the Golgi apparatus with ab184984 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human testis tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human pancreas tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry analysis of human colon tissue labeling PFG6 with ab184984 at a 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-PFD6 antibody (AB184984)

All lanes:

Western blot - Anti-PFD6 antibody (ab184984) at 1/500 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) whole cell lysate

Predicted band size: 14 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human PFDN6 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15212

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PFD6 also known as Prefoldin Subunit 6 is a component of the prefoldin complex. It has a molecular mass of approximately 15 kDa. PFD6 is expressed in various tissues including the heart liver and skeletal muscle. Mechanically PFD6 works as a molecular chaperone binding to newly synthesized polypeptides. It helps stabilize these nascent chains preventing premature folding and facilitating their transport to other complexes like the cytosolic chaperonin-containing TCP-1.

Biological function summary

PFD6 as part of the prefoldin complex assists in the correct folding of proteins necessary for cell function. The prefoldin complex acts as an assembly platform. It specifically interacts with cytoskeletal proteins such as actin and tubulin. This interaction is significant for maintaining cellular structure and dynamics. PFD6 therefore supports numerous cellular processes dependent on microtubule and actin filament formation.

Pathways

PFD6 participates in cellular maintenance processes. It plays a role in the protein folding and stabilization pathways working together with the cytosolic chaperonin CCT/TRiC complex. This interaction ensures that proteins involved in cell division and signaling like tubulins and actins are correctly folded. Additionally PFD6 connects to pathways involving cell motility and organization through stable support to the cytoskeleton.

PFD6 has links to certain neurodegenerative diseases and cancer. Aberrations in protein folding mechanisms can lead to accumulation of misfolded proteins contributing to neurodegenerative diseases like Alzheimer's. In cancer abnormal expression of PFD6 might affect cell structure and division due to its interactions with cytoskeletal elements like actin and tubulin. Research continues to explore how PFD6-related pathways influence these diseases highlighting potential therapeutic targets.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

See full target information PFDN6

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 47:52-62 PubMed36300673

2022

Prefoldin 6 promotes glioma progression via the AKT signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lianglei Jiang,Jun Liu

Clinical epigenetics 8:48 PubMed27152124

2016

DNA methylation profiling identifies novel markers of progression in hepatitis B-related chronic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Müjdat Zeybel,Sezgin Vatansever,Timothy Hardy,Ayşegül Akder Sarı,Fulya Cakalağaoğlu,Arzu Avcı,Gemma Louise Zeybel,Serçin Karahüseyinoğlu,Matthew Bashton,John C Mathers,Belkıs Ünsal,Jelena Mann

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PFD6 抗体

Anti-PFD6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunocytochemistry/ Immunofluorescence - Anti-PFD6 antibody (AB184984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFD6 antibody (AB184984)

Western blot - Anti-PFD6 antibody (AB184984)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise