Anti-PFAS 抗体 - N-terminal (ab186048)
Key features and details
- Rabbit polyclonal to PFAS - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PFAS antibody - N-terminal
PFAS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PFAS - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human PFAS aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_036525.1).
Database link: O15067 -
ポジティブ･コントロール
- Jurkat, HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab186048 was affinity purified using an epitope specific to PFAS immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186048の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/500 - 1/2500. Predicted molecular weight: 145 kDa.
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| 特記事項 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/500 - 1/2500. Predicted molecular weight: 145 kDa. |
ターゲット情報
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関連性
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 455093 Chimpanzee
- Entrez Gene: 102122705 Cynomolgus monkey
- Entrez Gene: 101150570 Gorilla
- Entrez Gene: 5198 Human
- Entrez Gene: 100173984 Orangutan
- Entrez Gene: 717046 Rhesus monkey
- Omim: 602133 Human
- SwissProt: O15067 Human
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別名
- PFAS antibody
- FGAM synthase antibody
- FGAMS antibody
see all
画像
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Western blot - Anti-PFAS antibody - N-terminal (ab186048)All lanes : Anti-PFAS antibody - N-terminal (ab186048) at 1 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : 293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 145 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PFAS antibody - N-terminal (ab186048)Detection of PFAS in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186048 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents rabbit IgG IP control.
Detection: Chemiluminescence with an exposure time of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab186048 は論文での使用が確認できていません。