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AB129183

Anti-PF4 抗体 [EPR7763]

Anti-PF4 antibody [EPR7763]

5

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal PF4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.

別名を表示する

CXCL4, SCYB4, PF4, Platelet factor 4, PF-4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (AB129183)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (AB129183)

ab129183 at a 1/100 dilution staining PF4 in paraffin embedded Human spleen tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-PF4 antibody [EPR7763] (AB129183)
  • WB

Unknown

Western blot - Anti-PF4 antibody [EPR7763] (AB129183)

All lanes:

Western blot - Anti-PF4 antibody [EPR7763] (ab129183) at 1/1000 dilution

Lane 1:

Human spleen lysates at 10 µg

Lane 2:

Human platelet lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11 kDa

false

OI-RD Scanning - Anti-PF4 antibody [EPR7763] (AB129183)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PF4 antibody [EPR7763] (AB129183)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

関連する標識済み抗体及び組成の異なる製品 (6)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR7763

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein Platelet Factor 4 (PF4) also known as CXCL4 is a member of the chemokine (C-X-C motif) ligand family. This small protein has a molecular weight of approximately 7.8 kDa. PF4 is mainly expressed in megakaryocytes and stored in the alpha granules of platelets. During platelet activation PF4 releases into the bloodstream where it interacts with other proteins and cell types. Researchers measure PF4 levels with methods such as PF4 ELISA to study its expression and functions under various physiological and pathological conditions.
Biological function summary

PF4 plays an important role in modulating the immune response and coagulation processes. PF4 is not part of a stable protein complex but can interact dynamically with heparin on the surface of endothelial cells and glycosaminoglycans. It inhibits the action of heparin promoting clot formation and angiogenesis. PF4 also attracts and activates different types of leukocytes and can regulate inflammatory processes influencing how the immune system responds to injury.

Pathways

PF4 significantly interacts with both the coagulation and inflammation pathways. In the coagulation pathway PF4 acts to neutralize heparin-like molecules thereby enhancing thrombosis. It works closely with proteins such as thrombin and fibrinogen contributing to the blood clot cascade. Within the inflammatory pathway PF4's chemokine activity helps direct leukocytes to sites of tissue damage or infection linking it to cytokines and other chemokines that mediate immune responses.

Researchers associate PF4 with conditions such as thrombosis and autoimmune pathologies. In thrombosis the overexpression of PF4 can lead to excessive clot formation pointing to its interaction with proteins like thrombin. PF4 also plays a role in heparin-induced thrombocytopenia (HIT) a disorder where the immune system mistakenly produces antibodies against heparin-PF4 complexes further indicating its direct involvement with immune and coagulation pathways.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation (PubMed : 29930254, PubMed : 9531587). Acts via different functional receptors including CCR1, CXCR3A or CXCR3B (PubMed : 18174362, PubMed : 29930254). Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling (PubMed : 18174362, PubMed : 24469069). Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence (PubMed : 9531587). Chemotactic for neutrophils and monocytes via CCR1 (PubMed : 29930254). Inhibits endothelial cell proliferation. In cooperation with toll-like receptor 8/TLR8, induces chromatin remodeling and activates inflammatory gene expression via the TBK1-IRF5 axis (PubMed : 35701499). In addition, induces myofibroblast differentiation and collagen synthesis in different precursor cells, including endothelial cells, by stimulating endothelial-to-mesenchymal transition (PubMed : 34986347). Interacts with thrombomodulin/THBD to enhance the activation of protein C and thus potentiates its anticoagulant activity (PubMed : 9395524).
See full target information PF4
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3480 PubMed40216753

2025

Small molecule BLVRB redox inhibitor promotes megakaryocytopoiesis and stress thrombopoiesis in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha M Nesbitt,Gian Luca Araldi,Lisa Pennacchia,Natalia Marchenko,Zahra Assar,Kendall M Muzzarelli,Rahul Raghavan Thekke Veedu,Brian Medel-Lacruz,Eunjeong Lee,Elan Z Eisenmesser,Dale F Kreitler,Wadie F Bahou

PloS one 17:e0270634 PubMed35749554

2022

Comparison of serum and plasma as a source of blood extracellular vesicles: Increased levels of platelet-derived particles in serum extracellular vesicle fractions alter content profiles from plasma extracellular vesicle fractions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Zhang,Toshihide Takeuchi,Akiko Takeda,Hideki Mochizuki,Yoshitaka Nagai

Cell chemical biology 29:1419-1433.e5 PubMed35738280

2022

A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kamalakannan Vijayan,Nadia Arang,Ling Wei,Robert Morrison,Rechel Geiger,K Rachael Parks,Adam J Lewis,Fred D Mast,Alyse N Douglass,Heather S Kain,John D Aitchison,Jarrod S Johnson,Alan Aderem,Alexis Kaushansky

Thrombosis and haemostasis 122:1115-1129 PubMed35253142

2022

Cucurbitacins Elicit Anti-Platelet Activity via Perturbation of the Cytoskeleton and Integrin Function.

Applications

Unspecified application

Species

Unspecified reactive species

Neline Kriek,Sophie H Nock,Tanya Sage,Badrija Khalifa,Alexander P Bye,Joanne L Mitchell,Steven Thomson,Mark G McLaughlin,Sarah Jones,Jonathan M Gibbins,Amanda J Unsworth

Journal of immunology research 2016:9276986 PubMed28053995

2016

CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction.

Applications

IHC

Species

Unspecified reactive species

Jing Li,Bin Liu,Yuan Shi,Ke-Liang Xie,Hai-Fang Yin,Lu-Nan Yan,Wan-Yee Lau,Guo-Lin Wang

The Journal of biological chemistry 291:9872-81 PubMed27006395

2016

Brain-derived Neurotrophic Factor in Megakaryocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Chacón-Fernández,Katharina Säuberli,Maria Colzani,Thomas Moreau,Cedric Ghevaert,Yves-Alain Barde
View all publications
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