Anti-PERP 抗体 (ab5986)

Thank you for your email and for keeping me updated about the situation.
I'm sorry to hear that ab5986 did not work well with the mouse samples, and it is possible that this antibody just doesn't work with mouse. We have unfortunately removed ab3945 from our catalog, but the discount code for ab48032 is still valid if you would like to try that antibody. The code expires in August, but if you need to extend that date please let me know and I'll be happy to issue you a new code.
Please let me know if you have any further questions or if there is anything else that we can do for you.

Thank you for your call today and for your interest in ab48032. Please see below for the details of your testing discount code, and let me know if you have any questions or if there is anything else that we can do for you.
DISCOUNT CODE: ***
Expiration date: August 24, 2012
This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab48032 using mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for the details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab5986. At this point I would like to make the following suggestions to help improve results as you are not seeing any bands. This antibody was characterized for use in Western blotting with A431 lysate and I strongly suggest using this as a positive control. I also suggest increasing the amount of primary that you are using to a dilution of 1:500 and incubating overnight at 4C. Also, you want to ensure that the protein transferred properly to the membrane (check this with Ponceau S) and check that your secondary is working properly. Is the secondary working with other primaries? You may also need to increase the incubation period and concentration of your secondary. I hope this helps. Please contact us again if you need additional assistance.

I'm sorry to hear you are having a problem with ab5986. I would like to suggest an overnight incubation of the antibody at 4C and maybe try a dilution of 1:300 as well. If you still have problems and your positive control (50ug of lysate per well) clearly shows that the antibody does not work please let me know and please send me an image of your blot and I will arrange for a replacement to be sent to you.

Thank you for getting back to us and providing us some more information about your recent results. We can see on the Western blot image that the 21 kDa band appears nicely, however there are two other non-specific bands as well. It is important to emphasize that ab5986 is a polyclonal antibody and it recognizes different epitopes. Therefore the antibody may recognize other proteins as well which share similar amino acid sequences. We would suggest perhaps reducing the concentration of the primary antibody and applying more stringent washings. Have you tested the secondary antibody alone? We hope this will help.