Anti-Peroxiredoxin 5 抗体 [EPR14529(B)]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Peroxiredoxin 5 with unpurified ab180587 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Peroxiredoxin 5 with purified ab180587 at 1 : 500 dilution (1.18 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IP

Unknown

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

ab180587 (Purified) at 1 : 30 dilution (2 µg) immunoprecipitating Peroxiredoxin 5 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+) : ab180587 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab180587 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

Predicted band size: 22 kDa

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• IP

Supplier Data

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Immunoprecipitation analysis of Peroxiredoxin 5 pulled down from HeLa cell lysates using ab180587 at 1/50 dilution.

All lanes:

Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/1000 dilution

All lanes:

HeLa cell immunoprecipitated with ab180587 at 1/50

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa,50 kDa

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• WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : PRDX5 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HepG2 whole cell lysate (20 μg)
Lane 4 : A549 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab180587 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180587 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. ab180587 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

Predicted band size: 22 kDa,44 kDa

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• WB

Supplier Data

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution

Lane 1:

HepG2 cell line lysate at 20 µg

Lane 2:

Jurkat cell line lysate at 20 µg

Lane 3:

A549 cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/20000 dilution

All lanes:

HeLa cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

• WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Human brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false

• WB

Lab

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (AB180587)

All lanes:

Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution

All lanes:

Mouse brain lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 17 kDa

false