Anti-Peroxiredoxin 4 抗体 [EPR15458(B)] (ab184167)
![Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.co.jp/ps/products/184/ab184167/Images/ab184167-393583-anti-peroxiredoxin-4-antibody-epr15458-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15458(B)] to Peroxiredoxin 4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Peroxiredoxin 4 antibody [EPR15458(B)]
Peroxiredoxin 4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15458(B)] to Peroxiredoxin 4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HepG2, K562, HEK293T and Jurkat cell lysates. IHC-P: Human ovarian carcinoma tissue, Mouse liver tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15458(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184167の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. -
配列類似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
細胞内局在
Cytoplasm. Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 10549 Human
- Entrez Gene: 53381 Mouse
- Entrez Gene: 85274 Rat
- Omim: 606506 Human
- SwissProt: Q13162 Human
- SwissProt: O08807 Mouse
- SwissProt: Q9Z0V5 Rat
- Unigene: 83383 Human
see all -
別名
- Antioxidant enzyme 372 antibody
- Antioxidant enzyme AOE372 antibody
- AOE37 2 antibody
see all
画像
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Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)All lanes : Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PRDX4 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab184167 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.
ab184167 Anti-Peroxiredoxin 4 antibody [EPR15458(B)] was shown to specifically react with Peroxiredoxin 4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266727 (knockout cell lysate ab257264) was used. Wild-type and Peroxiredoxin 4 knockout samples were subjected to SDS-PAGE. ab184167 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.co.jp/ps/products/184/ab184167/Images/ab184167-216571-anti-peroxiredoxin-4-antibody-epr15458b-immunohistochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Peroxiredoxin 4 with ab184167 at 1/250 dilution.
Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.co.jp/ps/products/184/ab184167/Images/ab184167-216570-anti-peroxiredoxin-4-antibody-epr15458b-immunohistochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)Immunohistochemical analysis of, paraffin-embedded Human ovarian carcinoma tissue labeling Peroxiredoxin 4 with ab184167 at 1/250 dilution. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.co.jp/ps/products/184/ab184167/Images/ab184167-216569-anti-peroxiredoxin-4-antibody-epr15458b-western-blot.png)
Western blot - Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)All lanes : Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
![Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)](https://www.abcam.co.jp/ps/products/184/ab184167/Images/ab184167-5-benefits-of-recombinant-antibodies.png)
Anti-Peroxiredoxin 4 antibody [EPR15458(B)] (ab184167)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (9)
ab184167 は 9 報の論文で使用されています。
- Cho E et al. Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration. Elife 12:N/A (2023). PubMed: 36735291
- Hao Y et al. Critical Role of the Sulfiredoxin-Peroxiredoxin IV Axis in Urethane-Induced Non-Small Cell Lung Cancer. Antioxidants (Basel) 12:N/A (2023). PubMed: 36829926
- Ding N et al. Peroxiredoxin IV plays a critical role in cancer cell growth and radioresistance through the activation of the Akt/GSK3 signaling pathways. J Biol Chem 298:102123 (2022). PubMed: 35697073
- Samtleben S et al. Astrocytes show increased levels of Ero1α in multiple sclerosis and its experimental autoimmune encephalomyelitis animal model. Eur J Neurosci 56:5177-5190 (2022). PubMed: 36083288
- Queiroz RF et al. Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation. Nat Commun 12:6626 (2021). PubMed: 34785665
- Lovatt M et al. Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells. Redox Biol 30:101417 (2020). PubMed: 31901729
- Zhu H et al. Dimethyl fumarate protects nucleus pulposus cells from inflammation and oxidative stress and delays the intervertebral disc degeneration. Exp Ther Med 20:269 (2020). PubMed: 33199994
- Stretton C et al. 2-Cys peroxiredoxin oxidation in response to hydrogen peroxide and contractile activity in skeletal muscle: A novel insight into exercise-induced redox signalling? Free Radic Biol Med 160:199-207 (2020). PubMed: 32784030
- Wang S et al. Single-Cell Transcriptomic Atlas of Primate Ovarian Aging. Cell 180:585-600.e19 (2020). PubMed: 32004457