Anti-Periostin 抗体 (ab152099)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Periostin antibody
Periostin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Periostin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Pig
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免疫原
Recombinant fragment, corresponding to a region within amino acids 604-807 of Human Periostin (Q15063).
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ポジティブ･コントロール
- A431, H1299, HeLa S3 and NIH 3T3 whole cell lysates; HeLa cells; Human gastric tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152099の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 93 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 93 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
組織特異性
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
配列類似性
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
翻訳後修飾
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
- Unigene: 721018 Human
- Unigene: 236067 Mouse
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別名
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab152099)Immunohistochemical analysis of paraffin-embedded Human gastric tissue labeling Periostin with ab152099 at 1/100 dilution.
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Western blot - Anti-Periostin antibody (ab152099)Anti-Periostin antibody (ab152099) at 1/1000 dilution + H1299 whole cell
lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Western blot - Anti-Periostin antibody (ab152099)Anti-Periostin antibody (ab152099) at 1/1000 dilution + NIH 3T3 whole cell
lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (ab152099)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Periostin with ab152099 at 1/200. Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab152099 は 3 報の論文で使用されています。
- Li X et al. Growth differentiation factor 11 mitigates cardiac radiotoxicity via activating AMPKa. Free Radic Res 55:176-185 (2021). PubMed: 33557626
- Zhang J et al. Integrative Analysis Reveals a miRNA-mRNA Regulatory Network and Potential Causative Agents in the Asthmatic Airway Epithelium. J Asthma Allergy 14:1307-1321 (2021). PubMed: 34744440
- Huang YW et al. A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder. Aging Dis 9:1043-1057 (2018). PubMed: 30574417