Anti-Periostin 抗体 (ab14041)
Key features and details
- Rabbit polyclonal to Periostin
- Suitable for: ELISA, ICC/IF, IHC-Fr, IHC-P
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Periostin antibody
Periostin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Periostin -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, IHC-Fr, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Chicken, Human -
免疫原
Recombinant fragment (His-tag) corresponding to Human Periostin (N terminal).
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ポジティブ･コントロール
- IHC-P: Human colon and normal skin tissues; Prostate cancer tissues. ICC/IF: HeLa cells.
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特記事項
Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilized form. To reconstitute, add 100 µL of deionized water and let the lyophilized antibody dissolve completely. The resulting concentration is 0.5 mg/mL. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: PBS, 0.58% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14041の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (3) |
Use at an assay dependent concentration.
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| IHC-Fr | (6) |
Use at an assay dependent concentration. PubMed: 19079190
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| IHC-P | (8) |
1/100 - 1/1000.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 19079190 |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
組織特異性
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
配列類似性
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
翻訳後修飾
Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 10631 Human
- Entrez Gene: 50706 Mouse
- Entrez Gene: 361945 Rat
- Omim: 608777 Human
- SwissProt: Q15063 Human
- SwissProt: Q62009 Mouse
- Unigene: 136348 Human
- Unigene: 721018 Human
see all -
別名
- Fasciclin-I like antibody
- MGC119510 antibody
- MGC119511 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)
Immunohistochemistry analysis of formalin-fixed paraffin-embedded human colon tissue section labeling Periostin with ab14041.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)Immunohistochemistry analysis of formalin-fixed paraffin-embedded human normal skin tissue sectionlabeling Periostin with ab14041. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)Tian et al. PLoS One. 2015 Mar 17;10(3):e0121502. doi: 10.1371/journal.pone.0121502. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/The periostin staining in prostate cancer and paired non-tumorous prostate tissues (TMA from JHU, 140 cases).
(A) The pathologists’ criteria for IHC scoring.(a) 0, undetectable; (b)1+, weak staining; (c) 2+, medium staining; (d) 3+, strong staining. Periostin was mainly stained in peritumoral stroma.
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Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (ab14041)ICC/IF image of ab14041 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (212)
ab14041 は 212 報の論文で使用されています。
- Daghrery A et al. Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues. Bioact Mater 19:268-281 (2023). PubMed: 35574052
- Okuno S et al. IL-31 and IL-31 receptor alpha in pemphigus: Contributors to more than just itch? J Dermatol 50:927-930 (2023). PubMed: 36651089
- Wenhua S et al. Cancer cell-derived novel periostin isoform promotes invasion in head and neck squamous cell carcinoma. Cancer Med 12:8510-8525 (2023). PubMed: 36691359
- Das S et al. ER stress induces upregulation of transcription factor Tbx20 and downstream Bmp2 signaling to promote cardiomyocyte survival. J Biol Chem 299:103031 (2023). PubMed: 36805334
- Sidorov M et al. Dual Targeting of EGFR and MTOR Pathways Inhibits Glioblastoma Growth by Modulating the Tumor Microenvironment. Cells 12:N/A (2023). PubMed: 36831214
