Anti-Perilipin 3/TIP47 抗体 (ab47639)
Key features and details
- Rabbit polyclonal to Perilipin 3/TIP47
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Perilipin 3/TIP47 antibody
Perilipin 3/TIP47 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Perilipin 3/TIP47 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse NIH/3T3, L1210 and EL4 cell lysates. Human CaCo-2, Daudi, HeLa, HepG2, K562, Jurkat, SK-N-SH and THP-1 cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47639の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 47, 46 kDa (predicted molecular weight: 47 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 47, 46 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network. -
配列類似性
Belongs to the perilipin family. -
細胞内局在
Cytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails. - Information by UniProt
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参照データベース
- Entrez Gene: 10226 Human
- Entrez Gene: 66905 Mouse
- Omim: 602702 Human
- SwissProt: O60664 Human
- SwissProt: Q9DBG5 Mouse
- Unigene: 140452 Human
- Unigene: 311696 Mouse
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別名
- 47 kDa mannose 6-phosphate receptor-binding protein antibody
- 47 kDa MPR binding protein antibody
- 47 kDa MPR-binding protein antibody
see all
画像
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Western blot - Anti-Perilipin 3/TIP47 antibody (ab47639)All lanes : Anti-Perilipin 3/TIP47 antibody (ab47639) at 1 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : L1210 (mouse lymphoblastic leukemia cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP)
Predicted band size: 47 kDa
Observed band size: 47 kDaDiluting buffer and concentration: 5% NFDM/TBST. Primary incubated for 1 hour at room temperature.
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Western blot - Anti-Perilipin 3/TIP47 antibody (ab47639)All lanes : Anti-Perilipin 3/TIP47 antibody (ab47639) at 1 µg/ml
Lane 1 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : SK-N-SH (human neuroblastoma cell line) cell lysate
Lane 8 : THP-1 (human monocytic leukemia cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.Diluting buffer and concentration: 5% NFDM/TBST. Primary incubated for 1 hour at room temperature.
Ab47639 can detect human isoforms 1, 3 and 4 which may account for the additional band.
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Western blot - Anti-Perilipin 3/TIP47 antibody (ab47639)Lane 1 : Anti-Perilipin 3/TIP47 antibody (ab47639) at 1 µg/ml
Lane 2 : Anti-Perilipin 3/TIP47 antibody (ab47639) at 2 µg/ml
All lanes : EL4 (mouse T cell lymphoblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDaDiluting buffer and concentration: 5% NFDM/TBST. Primary incubated for 1 hour at room temperature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab47639 は 7 報の論文で使用されています。
- Li G et al. Adipose tissue-specific ablation of Ces1d causes metabolic dysregulation in mice. Life Sci Alliance 5:N/A (2022). PubMed: 35459739
- Li X et al. Novel role of dynamin-related-protein 1 in dynamics of ER-lipid droplets in adipose tissue. FASEB J 34:8265-8282 (2020). PubMed: 32294302
- Lassen S et al. Perilipin-2 is critical for efficient lipoprotein and hepatitis C virus particle production. J Cell Sci 132:N/A (2019). PubMed: 30559250
- Vogt DA et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB, IP ; Human . PubMed: 23593007
- Cabodevilla AG et al. Cell survival during complete nutrient deprivation depends on lipid droplet-fueled ß-oxidation of fatty acids. J Biol Chem 288:27777-88 (2013). PubMed: 23940052
- Carr RM et al. Reduction of TIP47 improves hepatic steatosis and glucose homeostasis in mice. Am J Physiol Regul Integr Comp Physiol 302:R996-1003 (2012). WB . PubMed: 22378776
- García-Faroldi G et al. Polyamines are present in mast cell secretory granules and are important for granule homeostasis. PLoS One 5:e15071 (2010). WB ; Mouse . PubMed: 21151498