Anti-Perilipin 3/TIP47 抗体 (ab47638)
Key features and details
- Rabbit polyclonal to Perilipin 3/TIP47
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Perilipin 3/TIP47 antibody
Perilipin 3/TIP47 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Perilipin 3/TIP47 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Daudi cell lysate, Rat Liver tissue lysate; IHC-P: Rat Liver tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47638の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| IHC-P |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network. -
配列類似性
Belongs to the perilipin family. -
細胞内局在
Cytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails. - Information by UniProt
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参照データベース
- Entrez Gene: 10226 Human
- Entrez Gene: 316130 Rat
- Omim: 602702 Human
- SwissProt: O60664 Human
- Unigene: 140452 Human
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別名
- 47 kDa mannose 6-phosphate receptor-binding protein antibody
- 47 kDa MPR binding protein antibody
- 47 kDa MPR-binding protein antibody
see all
画像
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Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638)Lane 1 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/ml
Lane 2 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/ml
All lanes : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaDiluting buffer and concentration: 5% NFDM/TBST.
Primary incubated for 1 hour at room temperature.
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Western blot - Anti-Perilipin 3/TIP47 antibody (ab47638)Lane 1 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 0.5 µg/ml
Lane 2 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 1 µg/ml
Lane 3 : Anti-Perilipin 3/TIP47 antibody (ab47638) at 2 µg/ml
All lanes : Rat Liver tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaDiluting buffer and concentration: 5% NFDM/TBST.
Primary incubated for 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 3/TIP47 antibody (ab47638)ab47638 at 10 µg/ml staining Perilipin 3/TIP47 in rat liver tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab47638 は 8 報の論文で使用されています。
- Chen XJ et al. Lipid droplets' functional protein caveolin-2 is associated with lipid metabolism-related molecule FABP5 and EMT marker E-cadherin in oral epithelial dysplasia. J Clin Pathol 77:330-337 (2024). PubMed: 36854623
- Kim E et al. Porcine Follicular Fluid-Derived Exosome: The Pivotal Material for Porcine Oocyte Maturation in Lipid Antioxidant Activity. Int J Mol Sci 24:N/A (2023). PubMed: 37372955
- Zhang Y et al. Transcriptional analysis of the expression and prognostic value of lipid droplet-localized proteins in hepatocellular carcinoma. BMC Cancer 23:677 (2023). PubMed: 37464334
- Lamprou I et al. Suppressed PLIN3 frequently occurs in prostate cancer, promoting docetaxel resistance via intensified autophagy, an event reversed by chloroquine. Med Oncol 38:116 (2021). PubMed: 34410522
- Cheng X et al. Targeting DGAT1 Ameliorates Glioblastoma by Increasing Fat Catabolism and Oxidative Stress. Cell Metab 32:229-242.e8 (2020). PubMed: 32559414
- Negoita F et al. PNPLA3 variant M148 causes resistance to starvation-mediated lipid droplet autophagy in human hepatocytes. J Cell Biochem 120:343-356 (2019). PubMed: 30171718
- Laufman O et al. Viral Generated Inter-Organelle Contacts Redirect Lipid Flux for Genome Replication. Cell 178:275-289.e16 (2019). PubMed: 31204099
- Geng F et al. Inhibition of SOAT1 Suppresses Glioblastoma Growth via Blocking SREBP-1-Mediated Lipogenesis. Clin Cancer Res 22:5337-5348 (2016). PubMed: 27281560