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Anti-Perilipin 2 抗体 (ab52356)

Reviews (3)Q&A (1)References (38)
Western blot - Anti-Perilipin 2 antibody (ab52356)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 2 antibody (ab52356)
  • Immunocytochemistry - Anti-Perilipin 2 antibody (ab52356)

Key features and details

  • Rabbit polyclonal to Perilipin-2
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Perilipin 2 antibody [EPR3713] (ab108323)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Perilipin 2 antibody
    Perilipin-2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Perilipin-2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide corresponding to Mouse Perilipin-2 (C terminal).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab52356の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
WB (2)
Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/200.
特記事項
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P
1/200.

ターゲット情報

画像

  • Western blot - Anti-Perilipin 2 antibody (ab52356)
    Western blot - Anti-Perilipin 2 antibody (ab52356)
    Anti-Perilipin 2 antibody (ab52356) at 2 µg/ml + mouse liver lysate

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 2 antibody (ab52356)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perilipin 2 antibody (ab52356)

    Immunohistochemical analysis of mouse liver tissue staining Perilipin-2 using ab52356. 

  • Immunocytochemistry - Anti-Perilipin 2 antibody (ab52356)
    Immunocytochemistry - Anti-Perilipin 2 antibody (ab52356)

    Ab52356 staining Perilipin-2 in HeLa cells at 1/50 dilution. Hoechst 33258 was used as a nuclear counterstain. 

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (38)

ab52356 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab52356 は 38 報の論文で使用されています。

  • Fachada V  et al. Effects of Long-Term Physical Activity and BCAA Availability on the Subcellular Associations between Intramyocellular Lipids, Perilipins and PGC-1α. Int J Mol Sci 24:N/A (2023). PubMed: 36901715
  • Zhao W  et al. Identification of Ubr1 as an amino acid sensor of steatosis in liver and muscle. J Cachexia Sarcopenia Muscle 14:1454-1467 (2023). PubMed: 37057345
  • Lee J  et al. Microglial REV-ERBα regulates inflammation and lipid droplet formation to drive tauopathy in male mice. Nat Commun 14:5197 (2023). PubMed: 37626048
  • Zhu M  et al. AMPK Activator O304 Protects Against Kidney Aging Through Promoting Energy Metabolism and Autophagy. Front Pharmacol 13:836496 (2022). PubMed: 35308246
  • Jiang S  et al. Peroxisomal Fitness: A Potential Protective Mechanism of Fenofibrate against High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in Mice. Diabetes Metab J 46:829-842 (2022). PubMed: 35746892
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Rabbit polyclonal to Perilipin-2

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Lung
Blocking step
1% BSA and 1.5% Donkey Serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 11 2018

Western blot abreview for Rabbit polyclonal to Perilipin-2

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Mouse muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 25 2011

Western blot abreview for Rabbit polyclonal to Perilipin-2

 Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (AML-12 cell(liver cell line))
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Treatment
2uM Rosiglitazone for 24hrs
Specification
AML-12 cell(liver cell line)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 30 2009

Question

ab52356: IHC-Fr, IHC-P (untested) from mouse
first lot worked very nicely
second lot (GR49906-1) did work in ICC/IF with mouse primary cells, but neither in IHC-Fr nor IHC-P
Ab: 1/50, 1/100, 1/200 overnight 4 degree
block with BSA+goat serum, goat serum, or commercial blocking reagent
AR: citrate, proteinase K, trypsin or combination
detection: Alexa 488 or DAB (working otherwise)

ab23194: will test in mouse

Read More
Answer

Thank you for your phone call.

The free of charge replacement information for ab52356 can be found below. I am also sending youthe testing discount code for trying ab23194 in mouse - at the bottom of the email.


I am sorry to hear that our product ab52356 did not perform as before and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

====================================================

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab23194 in mouse. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I wish you the best of luck with your research.

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