AB16074

Anti-Perforin 抗体 [CB5.4]

Name: Anti-Perforin 抗体 [CB5.4] (ab16074) | Abcam
Brand: Abcam Limited
SKU: AB16074
Price: 68000 JPY
Availability: InStock
Rating: 4 (5 reviews)

Anti-Perforin antibody [CB5.4]

(5 Reviews)

(42 Publications)

Anti-Perforin antibody [CB5.4] (ab16074) is a rat monoclonal antibody detecting Perforin in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse.

- Over 30 publications
- Trusted since 2004

別名を表示する

Pfp, Prf1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

CB5.4

アイソタイプ

IgG2a

キャリアフリー

交差種

Mouse

アプリケーション

IHC-Fr, ICC, IP, WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Mouse Prf1 aa 50-550. The exact immunogen used to generate this antibody is proprietary information.

P10820

エピトープ

This antibody recognizes mouse perforin, region amino acids 402-534.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-Perforin antibody [CB5.4] (ab16074) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-Perforin [CB5.4] (ab16074) was first used in a scientific publication in 2004 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Perforin [CB5.4] (ab16074) has over 5 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.

Biological function summary

Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.

Pathways

Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.

Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 19446473, PubMed : 21037563, PubMed : 26306037, PubMed : 2783478, PubMed : 3261391, PubMed : 35148176, PubMed : 35705808, PubMed : 7520535, PubMed : 7526382, PubMed : 7972104, PubMed : 8164737). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 19446473, PubMed : 21037563, PubMed : 26306037, PubMed : 3261391, PubMed : 35148176, PubMed : 35705808, PubMed : 7526382, PubMed : 8164737). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 19446473, PubMed : 21037563, PubMed : 26306037, PubMed : 3261391, PubMed : 35148176, PubMed : 7526382, PubMed : 8164737). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (By similarity). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (PubMed : 35705808).

See full target information Prf1

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文献 (42)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 33:102045 PubMed40688666

2025

Dendritic cell membrane-based DCsLipo@MnO@siCTLA4@PD-1α nanomedicine for the treatment of Lynch syndrome-related colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Zhong,Pengcheng Wang,Jintian Wang,Yuejia Zhu,Liquan Chen,Yilong Qian,Xiang Huang,Kai Ye

Journal of experimental & clinical cancer research : CR 44:7 PubMed39773310

2025

Antibody-based delivery of interleukin-2 modulates the immunosuppressive tumor microenvironment and achieves cure in pancreatic ductal adenocarcinoma syngeneic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Carmine Carbone,Roberto De Luca,Emanuele Puca,Antonio Agostini,Alessia Caggiano,Lorenzo Priori,Annachiara Esposito,Serena Ascrizzi,Geny Piro,Lisa Salvatore,Francesco De Sanctis,Stefano Ugel,Vincenzo Corbo,Dario Neri,Giampaolo Tortora

Journal of cell communication and signaling 19:e12060 PubMed39720765

2024

M2-polarized tumor-associated macrophage-secreted exosomal lncRNA NEAT1 upregulates galectin-3 by recruiting KLF5 and promotes HCC immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yuan,Qigang Sun,Xiaodan Zhu,Bo Li,Yongping Zou,Zhehao Liu

Biomolecules & biomedicine 25:1184-1196 PubMed39652084

2024

miR-145-5p regulates hepatocellular carcinoma malignant advancement and immune escape via down-regulation of AcylCoA synthase ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Dingxue Wang,Wenqi Huang,Gao Li

Iranian journal of immunology : IJI 21:186-200 PubMed39319693

2024

JAK/STAT Signaling Pathway Mediates Anti-Tumor Immunity of CD8+ T Cells in Renal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Shao,Gang Deng,Guojun Wen,Xi Xie

Cancer research 84:4214-4232 PubMed39288081

2024

Combined Autophagy Inhibition and Dendritic Cell Recruitment Induces Antitumor Immunity and Enhances Immune Checkpoint Blockade Sensitivity in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Oyama,Kohei Nakata,Chikanori Tsutsumi,Masataka Hayashi,Bo Zhang,Yuki Mochida,Tomohiko Shinkawa,Kento Hirotaka,Pingshan Zhong,Satomi Date,Haizhen Luo,Akihiro Kubo,Nobuhiro Higashijima,Yutaka Yamada,Toshiya Abe,Noboru Ideno,Kazuhiro Koikawa,Chika Iwamoto,Naoki Ikenaga,Kenoki Ohuchida,Hideya Onishi,Takashi Morisaki,Keiji Kuba,Yoshinao Oda,Masafumi Nakamura

Molecular therapy : the journal of the American Society of Gene Therapy 32:3597-3617 PubMed39066478

2024

Specific targeting of cancer vaccines to antigen-presenting cells via an endogenous TLR2/6 ligand derived from cysteinyl-tRNA synthetase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeong Yun Kim,Seongmin Cho,Sang Bum Kim,Ee Chan Song,Wonchul Jung,Yun Gyeong Shin,Ji Hun Suh,Jihye Choi,Ina Yoon,Uijoo Kim,Hamin Ban,Sunkyo Hwang,Jeongwon Mun,Joohee Park,Nayoung Kim,Youngjin Lee,Myung Hee Kim,Sunghoon Kim

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308045 PubMed38520088

2024

EZH2 Inhibition Enhances PD-L1 Protein Stability Through USP22-Mediated Deubiquitination in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Huang,Qianqian Yin,Yuqing Wang,Xin Zhou,Yunyun Guo,Yuanjun Tang,Rui Cheng,Xiaotong Yu,Jie Zhang,Chen Huang,Zhanya Huang,Jianlin Zhang,Zhengyang Guo,Xiao Huo,Yan Sun,Yanfang Li,Hao Wang,Jianling Yang,Lixiang Xue

Nature communications 15:1487 PubMed38374152

2024

PIBF1 regulates trophoblast syncytialization and promotes cardiovascular development.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Geol Lee,Jung-Min Yon,Globinna Kim,Seul-Gi Lee,C-Yoon Kim,Seung-A Cheong,Hyun-Yi Kim,Jiyoung Yu,Kyunggon Kim,Young Hoon Sung,Hyun Ju Yoo,Dong-Cheol Woo,Jin Kyung Rho,Chang Hoon Ha,Chan-Gi Pack,Seak Hee Oh,Joon Seo Lim,Yu Mi Han,Eui-Ju Hong,Je Kyung Seong,Han-Woong Lee,Sang-Wook Lee,Ki-Up Lee,Chong Jai Kim,Sang-Yoon Nam,You Sook Cho,In-Jeoung Baek

Nature communications 14:7235 PubMed37945606

2023

Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiren Wang,Wenpan Li,Yanhao Jiang,Tuyen Ba Tran,Leyla Estrella Cordova,Jinha Chung,Minhyeok Kim,Georg Wondrak,Jennifer Erdrich,Jianqin Lu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Perforin 抗体 [CB5.4]

Anti-Perforin antibody [CB5.4]

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (42)

Abcam product promise