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AB89821

Anti-Perforin 抗体 [5B10]

Anti-Perforin antibody [5B10]

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(8 Publications)

Anti-Perforin antibody [5B10] (ab89821) is a mouse monoclonal antibody detecting Perforin in IHC-P. Suitable for Human.

- Trusted since 2010

別名を表示する

PFP, PRF1, Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [5B10] (AB89821)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [5B10] (AB89821)

Immunohistochemical analysis of human spleen labeling Perforin with ab89821 at 1 : 20 dilution. Secondary antibody was RTU PolyVue Plus HRP.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

5B10

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Recombinant Fragment Protein within Human PRF1. The exact immunogen used to generate this antibody is proprietary information.

P14222

特異性

This antibody is specific to a 70 kDa protein, which is a potent cytolytic pore-forming protein.

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代替製品

Primary Antibodies

AB238038

Anti-Perforin antibody [PRF1/2468]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [PRF1/2468] (AB238038)

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  • 0
Primary Antibodies

AB268229

Anti-Perforin antibody [PRF1/2470] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [PRF1/2470] - BSA and Azide free (AB268229)

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Primary Antibodies

AB268108

Anti-Perforin antibody [PRF1/2470]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [PRF1/2470] (AB268108)

  • 0
  • 0
Primary Antibodies

AB237888

Anti-Perforin antibody [PRF1/2468] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [PRF1/2468] - BSA and Azide free (AB237888)

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  • 0
Primary Antibodies

AB47225

Anti-Perforin antibody [B-D48] - BSA and Azide free

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Perforin antibody [B-D48] - BSA and Azide free (AB47225)

  • 4
  • 1
Primary Antibodies

AB47226

PE Anti-Perforin antibody [B-D48]

Flow Cytometry (Intracellular) - PE Anti-Perforin antibody [B-D48] (AB47226)

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  • 0
Primary Antibodies

AB86318

FITC Anti-Perforin antibody [B-D48]

  • 4
  • 1
Primary Antibodies

AB261727

Anti-Perforin antibody [EPR22829-42] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-Perforin antibody [EPR22829-42] - BSA and Azide free (AB261727)

  • 0
  • 0
Primary Antibodies

AB97305

Anti-Perforin antibody

Western blot - Anti-Perforin antibody (AB97305)

  • 0
  • 0
Primary Antibodies

AB16074

Anti-Perforin antibody [CB5.4]

  • 4
  • 5
Primary Antibodies

AB256453

Anti-Perforin antibody [EPR22829-42]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-Perforin antibody [EPR22829-42] (AB256453)

  • 0
  • 0

組合せ製品

Assay Kits

AB46068

Human Perforin ELISA Kit (PRF1)

ELISA - Human Perforin ELISA Kit (PRF1) (AB46068)

  • 0
  • 0
Primary Antibodies

AB216000

Anti-FOXP3 antibody [EPR15038-69] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR15038-69] - BSA and Azide free (AB216000)

  • 5
  • 1
Primary Antibodies

AB255598

Anti-Granzyme B antibody [EPR22645-206]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR22645-206] (AB255598)

  • 4
  • 3
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

What is this antibody validated in?
Anti-Perforin antibody [5B10] (ab89821) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Perforin also referred to as PRF1 is a pore-forming protein that plays an important role in immune response. It appears as a 67-kDa protein and is mainly expressed in cytotoxic T cells and natural killer (NK) cells. The function of perforin involves the formation of transmembrane pores in the membranes of target cells aiding in the delivery of pro-apoptotic molecules. This mechanism is essential for the destruction of virus-infected cells and tumor cells. Techniques like perforin IHC and perforin ELISA are suitable for detecting and quantifying this protein in various samples.
Biological function summary

Perforin helps the immune system respond effectively against infected or malignant cells. It works in conjunction with granzymes forming a complex that perforates target cell membranes allowing granzymes to enter and induce apoptosis. Perforin's role in immune surveillance prevents the propagation of harmful cells within the body. Deficiency or malfunction in perforin can lead to compromised immune function or immune cell dysregulation.

Pathways

Perforin integrates into the cytotoxic T lymphocyte (CTL) and NK cell-mediated cytotoxicity pathways. These pathways include critical interactions with proteins like granzymes and Fas ligand (FasL) which orchestrate the apoptosis of abnormal cells. Through these pathways perforin ensures the body's ability to identify and eliminate threats. The granule exocytosis pathway in particular heavily depends on perforin's activity to execute effective immune responses.

Mutations or defects in perforin are linked to familial hemophagocytic lymphohistiocytosis (FHL) and some types of cancer. These conditions arise when perforin's ability to function correctly is impaired. For instance in FHL defective perforin hampers the immune cells' ability to kill target cells leading to uncontrolled immune activation. Furthermore in the context of cancer the proper functioning of perforin alongside proteins like granzyme B is critical for the surveillance and elimination of tumor cells.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed : 20889983, PubMed : 21037563, PubMed : 24558045, PubMed : 9058810, PubMed : 9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed : 9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed : 20889983, PubMed : 21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed : 20038786, PubMed : 20225066, PubMed : 24558045, PubMed : 32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed : 24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity).
See full target information PRF1
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer research communications 5:358-368 PubMed39895413

2025

Phase Ib Study of Immunocytokine Simlukafusp Alfa (FAP-IL2v) Combined with Pembrolizumab for Treatment of Advanced and/or Metastatic Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Munoz-Couselo,Ainara Soria Rivas,Shahneen Sandhu,Georgina V Long,Miguel F Sanmamed,Anna Spreafico,Elizabeth Buchbinder,Mario Sznol,Hans Prenen,Alexander Fedenko,Mohammed Milhem,Ana Maria Arance Fernandez,Jean-Jacques Grob,Lev Demidov,Caroline Robert,Christin Habigt,Stefan Evers,Nassim Sleiman,David Dejardin,Caroline Ardeshir,Nicole Martin,Christophe Boetsch,Jehad Charo,Volker Teichgräber,Anton Kraxner,Nino Keshelava,Oliver Bechter

Clinical cancer research : an official journal of the American Association for Cancer Research 30:5540-5547 PubMed39422604

2024

Phase Ib Study of the Immunocytokine Simlukafusp Alfa (FAP-IL2v) in Combination with Cetuximab in Patients with Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron R Hansen,Carlos A Gomez-Roca,Debbie G J Robbrecht,Loic Verlingue,Antoine Italiano,Julie E Bauman,Neeltje Steeghs,Hans Prenen,Jérôme Fayette,James Spicer,Jiaxin Niu,Christin Habigt,Meike Schneider,Stefan Evers,Nassim Sleiman,David Dejardin,Caroline Ardeshir,Daniela Schmid,Christophe Boetsch,Jehad Charo,Anton Kraxner,Volker Teichgräber,Nino Keshelava,Marcelo R Bonomi

Frontiers in immunology 15:1352632 PubMed39035007

2024

Investigating the complex interplay between fibroblast activation protein α-positive cancer associated fibroblasts and the tumor microenvironment in the context of cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Anton Kraxner,Franziska Braun,Wei-Yi Cheng,Tai-Hsien Ou Yang,Shweta Pipaliya,Marta Canamero,Emilia Andersson,Suzana Vega Harring,Sebastian Dziadek,Ann-Marie E Bröske,Maurizio Ceppi,Tamara Tanos,Volker Teichgräber,Jehad Charo

Neurology(R) neuroimmunology & neuroinflammation 10: PubMed37080596

2023

Effects of a Small-Molecule Perforin Inhibitor in a Mouse Model of CD8 T Cell-Mediated Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Gonzalez-Fierro,Coralie Fonte,Eloïse Dufourd,Vincent Cazaentre,Sidar Aydin,Britta Engelhardt,Rachel R Caspi,Biying Xu,Guillaume Martin-Blondel,Julie A Spicer,Joseph A Trapani,Jan Bauer,Roland S Liblau,Chloé Bost

Cancer cell 40:1600-1618.e10 PubMed36423635

2022

Cancer immunotherapies transition endothelial cells into HEVs that generate TCF1 T lymphocyte niches through a feed-forward loop.

Applications

Unspecified application

Species

Unspecified reactive species

Yichao Hua,Gerlanda Vella,Florian Rambow,Elizabeth Allen,Asier Antoranz Martinez,Marie Duhamel,Akira Takeda,Sirpa Jalkanen,Steffie Junius,Ann Smeets,David Nittner,Stefanie Dimmeler,Thomas Hehlgans,Adrian Liston,Francesca Maria Bosisio,Giuseppe Floris,Damya Laoui,Maija Hollmén,Diether Lambrechts,Pascal Merchiers,Jean-Christophe Marine,Susan Schlenner,Gabriele Bergers

JFMS open reports 5:2055116919863080 PubMed31321069

2019

Blastic natural killer cell lymphoma/leukaemia in a cat.

Applications

Unspecified application

Species

Unspecified reactive species

Miyuki Hirabayashi,James K Chambers,Mei Sugawara,Aki Ohmi,Hajime Tsujimoto,Hiroyuki Nakayama,Kazuyuki Uchida

Journal of immunology (Baltimore, Md. : 1950) 192:2120-32 PubMed24489088

2014

CD4+ T cells contain early extrapulmonary tuberculosis (TB) dissemination and rapid TB progression and sustain multieffector functions of CD8+ T and CD3- lymphocytes: mechanisms of CD4+ T cell immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Yao,Dan Huang,Crystal Y Chen,Lisa Halliday,Richard C Wang,Zheng W Chen

Cancer research 73:1892-9 PubMed23302230

2013

Focused ultrasound delivers targeted immune cells to metastatic brain tumors.

Applications

IHC-P

Species

Human

Ryan Alkins,Alison Burgess,Milan Ganguly,Giulio Francia,Robert Kerbel,Winfried S Wels,Kullervo Hynynen
View all publications
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