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PerCP/Cy5.5® Anti-CD45 抗体 [MEM-28] (ab157309)

Reviews (1) Submit a question References (1)
Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309)

    Key features and details

    • PerCP/Cy5.5® Mouse monoclonal [MEM-28] to CD45
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 647 APC APC/Cy7® Biotin FITC PE PE/Cy7® PE/DyLight™ 594 PerCP Unconjugated

    製品の概要

    • 製品名

      PerCP/Cy5.5® Anti-CD45 antibody [MEM-28]
      CD45 一次抗体 製品一覧

    • 製品の詳細

      PerCP/Cy5.5® Mouse monoclonal [MEM-28] to CD45

    • 由来種

      Mouse

    • 標識

      PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

    • 特異性

      ab157309 reacts with all alternative forms of Human CD45, expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Tissue, cells or virus corresponding to Human CD45. Human thymocytes and T lymphocytes.

    • ポジティブ･コントロール

      • Flow Cyt: Human peripheral blood cells.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab157309の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt (1)
    Use 4µl for 106 cells.

    Or use 100 µl of whole blood.

    ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    特記事項
    Flow Cyt
    Use 4µl for 106 cells.

    Or use 100 µl of whole blood.

    ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

    • 関連疾患

      Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
      Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

    • 配列類似性

      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.

    • ドメイン

      The first PTPase domain interacts with SKAP1.

    • 翻訳後修飾

      Heavily N- and O-glycosylated.

    • 細胞内局在

      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt

    • 参照データベース

      • 別名

        • B220 antibody
        • CD 45 antibody
        • CD45 antibody
        • CD45 antigen antibody
        • CD45R antibody
        • GP180 antibody
        • L-CA antibody
        • LCA antibody
        • Leukocyte common antigen antibody
        • loc antibody
        • Ly-5 antibody
        • LY5 antibody
        • Ly5, homolog of antibody
        • Lyt-4 antibody
        • OTTHUMP00000033813 antibody
        • OTTHUMP00000033816 antibody
        • OTTHUMP00000033817 antibody
        • OTTHUMP00000038574 antibody
        • Protein tyrosine phosphatase receptor type c polypeptide antibody
        • Protein tyrosine phosphatase, receptor type C antibody
        • protein tyrosine phosphatase, receptor type, C antibody
        • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
        • Ptprc antibody
        • PTPRC_HUMAN antibody
        • Receptor-type tyrosine-protein phosphatase C antibody
        • T200 antibody
        • T200 glycoprotein antibody
        • T200 leukocyte common antigen antibody
        see all

      画像

      • Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309)
        Flow Cytometry - PerCP/Cy5.5® Anti-CD45 antibody [MEM-28] (ab157309)

        Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) PerCP-CyTM5.5.

      参考文献 (1)

      ab157309 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab157309 は 1 報の論文で使用されています。

      • Wu H  et al. Regenerative potential of human nucleus pulposus resident stem/progenitor cells declines with ageing and intervertebral disc degeneration. Int J Mol Med 42:2193-2202 (2018). PubMed: 30015833

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Flow Cytometry abreview for Anti-CD45 antibody [MEM-28] (PerCP/Cy5.5®)

       Excellent
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (PBMC)
      Specification
      PBMC
      Preparation
      Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient isolation
      Sample buffer: PBS, Ficoll-Hypaque
      Permeabilization
      No
      Gating Strategy
      lymphocytes
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Apr 09 2013

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