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AB210324

PerCP/Cy5.5® Anti-CD4 抗体 [OKT4]

PerCP/Cy5.5® Anti-CD4 antibody [OKT4]

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(1 Publication)

Anti-CD4 antibody [OKT4] conjugated to PerCP/Cy5.5® - suitable for flow cytometry, and reacts with human samples. Clone [OKT4] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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別名を表示する

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images
Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [OKT4] (AB210324)
  • Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD4 antibody [OKT4] (AB210324)

Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD4 with 5 μl (0.25 μg) ab210324 (solid line), compared with 0.25 μg of PerCP/Cy5.5® Mouse IgG2b isotype control (dashed line).

関連する標識済み抗体及び組成の異なる製品 (5)

  • 660 APC

    APC Anti-CD4 antibody [OKT4]

  • 519 FITC

    FITC Anti-CD4 antibody [OKT4]

  • 578 PE

    PE Anti-CD4 antibody [OKT4]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD4 antibody [OKT-4]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD4 antibody [OKT-4]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

OKT4

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

標識

PerCP/Cy5.5®

励起波長/蛍光波長

Ex: 482nm, Em: 695nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>The antibody has been diluted for use at 5 μl (0.25 μg) per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 μl.</p>" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

製品の詳細

This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
ab210324 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Store in the dark

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 16:1403-1410 PubMed30112067

2018

Effect of TGF-β1 on blood CD4CD25 regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Hu,Weibo Qi,Li Sun,Hui Zhou,Biliu Zhou,Zhiping Yang
View all publications

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