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AB157317

PerCP/Cy5.5® Anti-CD3 抗体 [UCHT1], prediluted

PerCP/Cy5.5® Anti-CD3 antibody [UCHT1]

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(5 Publications)

Anti-CD3 antibody [UCHT1] conjugated to PerCP/Cy5.5® - suitable for flow cytometry, intracellular flow cytometry, and reacts with human samples. Clone [UCHT1] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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別名を表示する

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - PerCP/Cy5.5® Anti-CD3 antibody [UCHT1] (AB157317)
  • Flow Cyt

Lab

Flow Cytometry - PerCP/Cy5.5® Anti-CD3 antibody [UCHT1] (AB157317)

Analysis by flow cytometry of ab157317 staining CD3 in human peripheral blood cells.

関連する標識済み抗体及び組成の異なる製品 (13)

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  • 519 FITC

    FITC Anti-CD3 epsilon antibody [UCHT1]

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  • 578 PE

    PE Anti-CD3 epsilon antibody [UCHT1]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD3 antibody [UCHT1]

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    PE/Cy7® Anti-CD3 antibody [UCHT1]

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  • 387 mFluor™ UV375

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    violetFluor™ 450 Anti-CD3 antibody [UCHT1]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

UCHT1

アイソタイプ

IgG1

標識

PerCP/Cy5.5®

励起波長/蛍光波長

Ex: 482nm, Em: 695nm

キャリアフリー

No

交差種

Human, Primates

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

特異性

ab157317 reacts with the epsilon chain of the CD3 complex.

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代替製品

Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

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Primary Antibodies

AB16044

Anti-CD3 antibody

Immunoprecipitation - Anti-CD3 antibody (AB16044)

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  • 2
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

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  • 80
Primary Antibodies

AB21703

Anti-CD3 epsilon antibody [SP7], prediluted

BOND RX™ Validated|RabMAb|Lab Essentials|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7], prediluted (AB21703)

  • 5
  • 1
Primary Antibodies

AB245731

Anti-CD3 antibody [SP162] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - BSA and Azide free (AB245731)

  • 5
  • 3
Primary Antibodies

AB246799

Anti-CD3 antibody [SP162] - Low endotoxin, Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] - Low endotoxin, Azide free (AB246799)

  • 0
  • 0
Primary Antibodies

AB5690

Anti-CD3 epsilon antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690)

  • 4
  • 46

組合せ製品

Primary Antibodies

AB305048

Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [CAL66] (AB305048)

  • 5
  • 1
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB237456

Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-NCAM1 antibody [EP2567Y] (AB237456)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>(or 100 μl of whole blood).</p><p><a href='/products/primary-antibodies/percp-cy55-mouse-igg1-kappa-monoclonal-mopc-21-isotype-control-ab157226'>ab157226</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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製品の詳細

Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 40:101831 PubMed38039947

2023

Silencing of PD-1 combined with EBV-specific killer T cells for the treatment of EBV-associated B lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaping Wang,Zhijuan Xu,Yanli Lai,Yanli Zhang,Ping Zhang,Qitian Mu,Shujun Yang,Yongcheng Sun,Lixia Sheng,Guifang Ouyang

The Journal of experimental medicine 204:2537-44 PubMed17923503

2007

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Applications

Unspecified application

Species

Unspecified reactive species

Gabrielle M Siegers,Mahima Swamy,Edgar Fernández-Malavé,Susana Minguet,Sylvia Rathmann,Alberto C Guardo,Verónica Pérez-Flores,Jose R Regueiro,Balbino Alarcón,Paul Fisch,Wolfgang W A Schamel

The New England journal of medicine 354:1913-21 PubMed16672702

2006

Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric Rieux-Laucat,Claire Hivroz,Annick Lim,Véronique Mateo,Isabelle Pellier,Françoise Selz,Alain Fischer,Françoise Le Deist

Proceedings of the National Academy of Sciences of 101:16268-73 PubMed15534202

2004

Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly L Arnett,Stephen C Harrison,Don C Wiley

Journal of immunology (Baltimore, Md. : 1950) 161:1113-22 PubMed9686569

1998

Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

S le Gouvello,V Manceau,A Sobel
View all publications
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