AB234265

PerCP/Cy5.5® Anti-CD105 抗体 [MEM-229]

Name: PerCP/Cy5.5® Anti-CD105 抗体 [MEM-229] (ab234265) | Abcam
Brand: Abcam Limited
SKU: AB234265
Price: 72000 JPY
Availability: InStock

PerCP/Cy5.5® Anti-CD105 antibody [MEM-229]

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(4 Publications)

Anti-CD105 antibody [MEM-229] conjugated to PerCP/Cy5.5® - suitable for flow cytometry, and reacts with human samples. Clone [MEM-229] is a mouse monoclonal antibody cited in 64 publications.

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別名を表示する

CD105, END, ENG, Endoglin

2 Images

Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (AB234265)

Flow Cyt

Unknown

Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (AB234265)

Surface staining of CD105 on Huvec cells with ab234265 at 1/25 dilution

Flow Cyt

Supplier Data

Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (AB234265)

Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell line) cells, labeling CD105 with ab234265 at 4 μL per 10⁶ cells (red) compared to a negative control (blue). Gated on HUVEC cells.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM-229

アイソタイプ

IgG2a

標識

PerCP/Cy5.5®

励起波長/蛍光波長

Ex: 482nm, Em: 695nm

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Recombinant Virus within Human ENG. The exact immunogen used to generate this antibody is proprietary information.

P17813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μL whole blood)" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μL whole blood)" }, "Horse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "(or 100 μL whole blood)" }, "Pig": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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製品の詳細

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Size-exclusion chromatography

精製に関する特記事項

The purified antibody is conjugated with tandem dye PerCP-Cy™5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

バッファー組成

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze|Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).

See full target information ENG

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35406690

2022

Single-Cell Molecular Characterization to Partition the Human Glioblastoma Tumor Microenvironment Genetic Background.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Lessi,Sara Franceschi,Mariangela Morelli,Michele Menicagli,Francesco Pasqualetti,Orazio Santonocito,Carlo Gambacciani,Francesco Pieri,Filippo Aquila,Paolo Aretini,Chiara Maria Mazzanti

Heliyon 7:e07996 PubMed34589625

2021

Mesenchymal stem cells act as stimulators of neurogenesis and synaptic function in a rat model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Doshmanziari,Sadegh Shirian,Mohammad-Reza Kouchakian,Seyedeh Farzaneh Moniri,Saranaz Jangnoo,Niloofar Mohammadi,Fariba Zafari

Journal of tissue engineering and regenerative medicine 15:487-502 PubMed33734580

2021

Regulating effect of Circ_ATRNL1 on the promotion of SOX9 expression to promote chondrogenic differentiation of hAMSCs mediated by MiR-145-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhu,Qiwei Fu,Jiahua Shao, Jinhui Peng,Qirong Qian,Yiqin Zhou, Yi Chen

International journal of molecular medicine 43:1395-1405 PubMed30628649

2019

Study of the cytological features of bone marrow mesenchymal stem cells from patients with neuromyelitis optica.

Applications

Unspecified application

Species

Unspecified reactive species

Chunsheng Yang,Yang Yang,Li Ma,Guang-Xian Zhang,Fu-Dong Shi,Yaping Yan,Guoqiang Chang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

PerCP/Cy5.5® Anti-CD105 抗体 [MEM-229]

PerCP/Cy5.5® Anti-CD105 antibody [MEM-229]

Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (AB234265)

Flow Cytometry - PerCP/Cy5.5® Anti-CD105 antibody [MEM-229] (AB234265)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise