Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

PerCP Anti-CD8 抗体 [MEM-31] (ab65949)

Reviews (1) Submit a question References (2)

Key features and details

  • PerCP Mouse monoclonal [MEM-31] to CD8
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PerCP. Ex: 482nm, Em: 675nm
  • Isotype: IgG2a

製品の概要

  • 製品名

    PerCP Anti-CD8 antibody [MEM-31]
    CD8 一次抗体 製品一覧

  • 製品の詳細

    PerCP Mouse monoclonal [MEM-31] to CD8

  • 由来種

    Mouse

  • 標識

    PerCP. Ex: 482nm, Em: 675nm

  • 特異性

    ab65949 recognizes a conformationally-dependent epitope of CD8. The antibody does not react with formaldehyde-fixed cells and is negative in Western Blotting.

  • アプリケーション

    適用あり: Flow Cytmore details
    適用なし: WB

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD8. Crude thymus membrane fraction

  • ポジティブ･コントロール

    • human blood

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab65949の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (1)
Use 10µl for 106 cells.

ab110422 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Use Neat: 10µl for 106 cells, or 100µl whole blood.

Is unsuitable for applications using formaldehyde fixed cells.
特記事項
Flow Cyt
Use 10µl for 106 cells.

ab110422 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Use Neat: 10µl for 106 cells, or 100µl whole blood.

Is unsuitable for applications using formaldehyde fixed cells.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

  • 関連疾患

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

  • 配列類似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻訳後修飾

    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.

    • 別名

      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    参考文献 (2)

    ab65949 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab65949 は 2 報の論文で使用されています。

    • Abu N  et al. Subchronic toxicity, immunoregulation and anti-breast tumor effect of Nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L. BMC Complement Altern Med 18:31 (2018). PubMed: 29374471
    • Linnebacher M  et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol 2010:841451 (2010). PubMed: 20339516

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Flow Cytometry abreview for Anti-CD8 antibody [MEM-31] (PerCP)

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Permeabilization
    No
    Sample
    Human Cell (whole blood)
    Specification
    whole blood
    Gating Strategy
    lymphocytes
    Preparation
    Cell harvesting/tissue preparation method: Ficoll-Hypaque
    Sample buffer: PBS
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 19 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com