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PerCP Anti-CD45 抗体 [MEM-28] (ab65952)

Submit a review Q&A (1)References (4)

Key features and details

  • PerCP Mouse monoclonal [MEM-28] to CD45
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PerCP. Ex: 482nm, Em: 675nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC APC/Cy7® Biotin FITC PE PE/Cy7® PE/DyLight™ 594 PerCP/Cy5.5® Unconjugated

製品の概要

  • 製品名

    PerCP Anti-CD45 antibody [MEM-28]
    CD45 一次抗体 製品一覧

  • 製品の詳細

    PerCP Mouse monoclonal [MEM-28] to CD45

  • 由来種

    Mouse

  • 標識

    PerCP. Ex: 482nm, Em: 675nm

  • 特異性

    ab65952 reacts with all alternative forms of human CD45 antigen.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human CD45. Human thymocytes and T lymphocytes

  • ポジティブ･コントロール

    • Flow Cyt: Human blood cells.

  • 特記事項

    The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab65952の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of neat solution for 10^6 cells or 100ul of whole blood ab118658 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
Flow Cyt
Use at an assay dependent concentration.

Use 10ul of neat solution for 10^6 cells or 100ul of whole blood ab118658 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

  • 関連疾患

    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.

  • 配列類似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • ドメイン

    The first PTPase domain interacts with SKAP1.

  • 翻訳後修飾

    Heavily N- and O-glycosylated.

  • 細胞内局在

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    参考文献 (4)

    ab65952 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab65952 は 4 報の論文で使用されています。

    • Gholizadeh-Ghaleh Aziz S  et al. Human amniotic fluid stem cells (hAFSCs) expressing p21 and cyclin D1 genes retain excellent viability after freezing with (dimethyl sulfoxide) DMSO. Bosn J Basic Med Sci 19:43-51 (2019). PubMed: 29688163
    • Shao B  et al. Regulatory effects of miRNA-181a on FasL expression in bone marrow mesenchymal stem cells and its effect on CD4+T lymphocyte apoptosis in estrogen deficiency-induced osteoporosis. Mol Med Rep 18:920-930 (2018). PubMed: 29845202
    • Gholizadeh-Ghaleh Aziz S  et al. Isolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells. PLoS One 11:e0158281 (2016). Flow Cyt ; Human . PubMed: 27434028
    • Sadallah S  et al. Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells. J Immunol 186:6543-52 (2011). Flow Cyt ; Human . PubMed: 21525379

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    When this antibody was tested in flow cytometry, how long was the antibody incubated with the cells and at what temperature?

    Read More
    Answer

    Thank you very much for your phone call yesterday and for your patience while I have been in touch with the lab.

    Ab65952 was tested in flow cytometry with a primary incubation time of 20-30 minutes at room temperature in the dark.

    I hope this information will be useful, but if you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

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