Anti-PER1 抗体 [2715C2] (ab58886)
![Western blot - Anti-PER1 antibody [2715C2] (ab58886)](https://www.abcam.co.jp/ps/products/58/ab58886/Images/ab58886.gif "Western blot - Anti-PER1 antibody [2715C2] (ab58886)")
Key features and details
- Mouse monoclonal [2715C2] to PER1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-PER1 antibody [2715C2]
PER1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2715C2] to PER1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Human
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免疫原
Recombinant fragment of Human PER1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2715C2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58886の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 136 kDa. Antibody has only been tested on the recombinant fragment (immunogen). We have no data regarding endogenous samples.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 136 kDa. Antibody has only been tested on the recombinant fragment (immunogen). We have no data regarding endogenous samples. |
ターゲット情報
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機能
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
NPAS2-BMAL1
BMAL2-induced transactivation. -
組織特異性
Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney. -
配列類似性
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻訳後修飾
Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. -
細胞内局在
Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. - Information by UniProt
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参照データベース
- Entrez Gene: 5187 Human
- Omim: 602260 Human
- SwissProt: O15534 Human
- Unigene: 445534 Human
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別名
- Circadian clock protein PERIOD 1 antibody
- Circadian clock protein PERIOD1 antibody
- Circadian pacemaker protein Rigui antibody
see all
画像
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Western blot - Anti-PER1 antibody [2715C2] (ab58886)Anti-PER1 antibody [2715C2] (ab58886) + immunising recombinant fragment
Predicted band size: 136 kDa
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab58886 は論文での使用が確認できていません。
