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Anti-PEP1 抗体 [18C8BC7AD10] (ab113690)

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Western blot - Anti-PEP1 antibody [18C8BC7AD10] (ab113690)

    Key features and details

    • Mouse monoclonal [18C8BC7AD10] to PEP1
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG2c

    製品の概要

    • 製品名

      Anti-PEP1 antibody [18C8BC7AD10]

    • 製品の詳細

      Mouse monoclonal [18C8BC7AD10] to PEP1

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Saccharomyces cerevisiae

    • 免疫原

      Recombinant full length protein. This information is considered to be commercially sensitive.

    • ポジティブ･コントロール

      • Yeast lysate

    • 特記事項

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C.

    • バッファー

      pH: 7.5
      Preservative: 0.02% Sodium azide
      Constituent: HEPES buffered saline
    • Concentration information loading...

    • 精製度

      Proprietary Purification

    • 特記事項（精製）

      Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.

    • ポリ/モノ

      モノクローナル

    • クローン名

      18C8BC7AD10

    • アイソタイプ

      IgG2c

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab113690の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).
    特記事項
    WB
    Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).

    ターゲット情報

    • 機能

      Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.

    • 配列類似性

      Belongs to the VPS10-related sortilin family.
      Contains 10 BNR repeats.

    • ドメイン

      The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.

    • 細胞内局在

      Golgi apparatus > trans-Golgi network membrane. Prevacuolar compartment membrane. Cycles between the Golgi apparatus and the prevacuolar compartments.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Carboxypeptidase Y receptor antibody
        • Carboxypeptidase Y-deficient protein 1 antibody
        • CPY receptor antibody
        • pep1 antibody
        • Sortilin VPS10 antibody
        • Vacuolar carboxypeptidase sorting receptor VPS10 antibody
        • Vacuolar protein sorting-associated protein 10 antibody
        • Vacuolar protein sorting/targeting protein VPS10 antibody
        • Vacuolar protein-targeting protein 1 antibody
        • VPS10_YEAST antibody
        see all

      画像

      • Western blot - Anti-PEP1 antibody [18C8BC7AD10] (ab113690)
        Western blot - Anti-PEP1 antibody [18C8BC7AD10] (ab113690)
        Anti-PEP1 antibody [18C8BC7AD10] (ab113690) + Yeast whole cell lysate at 15 µg

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 177 kDa

      参考文献 (2)

      ab113690 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab113690 は 2 報の論文で使用されています。

      • Nightingale DJH  et al. A Protocol to Map the Spatial Proteome Using HyperLOPIT in Saccharomyces cerevisiae. Bio Protoc 9:e3303 (2019). PubMed: 33654815
      • Aufschnaiter A  et al. The Coordinated Action of Calcineurin and Cathepsin D Protects Against a-Synuclein Toxicity. Front Mol Neurosci 10:207 (2017). PubMed: 28713240

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