AB325639

Anti-Pentraxin 3/PTX3 antibody [EPR30744-540]

RabMAb
Recombinant
20ul selling size
Advanced Validation
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Rabbit Recombinant Monoclonal Pentraxin 3/PTX3 antibody. Suitable for IHC-P, WB, mIHC and reacts with Transfected cell line - Human, Mouse samples.

別名を表示する

Tsg14, Ptx3, Pentraxin-related protein PTX3, Pentaxin-related protein PTX3, Tumor necrosis factor-inducible gene 14 protein, TSG-14

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a human PTX3 expression vector containing a Myc-His tag and (B) HEK-293T transfected with empty vector containing a Myc-His tag tissue labeling Pentraxin 3/PTX3 with ab325639 at 1/2000 (0.254 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a human PTX3 expression vector containing a Myc-His tag, no staining on (B) HEK-293T transfected with empty vector containing a Myc-His tag. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Pentraxin 3/PTX3 with ab325639 at 1/100 (5.07 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of mouse cardiac muscle (PMID : 31209269; PMID : 23372656). The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Immunohistochemical analysis of paraffin-embedded (A) 3T3-L1 (mouse embryonic fibroblast) cell pellet (B) NIH/3T3 (mouse embryonic fibroblast) cell pellet (C) B16-F10 (mouse skin melanoma cell) cell pellet (D) Neuro-2a (mouse neuroblastoma cell) cell pellet labeling Pentraxin 3/PTX3 with ab325639 at 1/100 (5.07 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) 3T3-L1 cell pellet, (B) NIH/3T3 cell pellet, no staining on (C) B16-F10 cell pellet, (D) Neuro-2a cell pellet. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Pentraxin 3/PTX3 with ab325639 at 1/100 (5.07 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of mouse skeletal muscle (PMID : 27900389). The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

mIHC

Lab

Multiplex immunohistochemistry - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung treated with lps (1ug/ml 16h)+bfa (1ug/ml 16h) (+) ex vivo tissue staining Pentraxin 3/PTX3 with ab325639 at a 1 : 100 (5.07 µg/ml) dilution, ab283654 anti-CD68 used at 1 : 100 (4.15 µg/ml) dilution.

Panel A : merged staining of anti-Pentraxin 3 (green; Opal™520), anti-E CD68 (magenta; Opal™570) on mouse lung treated with LPS (1ug/ml 16h)+BFA (1ug/ml 16h) ex vivo.
Panel B : anti-Pentraxin 3 staining stroma cells in mouse lung treated with LPS (1ug/ml 16h)+BFA (1ug/ml 16h) ex vivo.
Panel C : anti-CD68 staining macrophages in mouse lung treated with LPS (1ug/ml 16h)+BFA (1ug/ml 16h) ex vivo.
Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325639 and ab283654 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : B16-F10.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (ab325639)

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

B16-F10 (mouse skin melanoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Neuro-2a.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (ab325639)

Lane 1:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 50 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 6s

Lab

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (AB325639)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Pentraxin 3/PTX3 antibody [EPR30744-540] (ab325639)

All lanes:

Mouse skeletal muscle tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 180s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

WB, mIHC, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/100", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.

See full target information Ptx3

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pentraxin 3/PTX3 antibody [EPR30744-540]